The Type III Secretion System plasmid pPHDPT3 of Photobacterium damselae subsp. piscicida is stable in Australian isolates due to conserved non-repetitive genomic architecture.

Oleksandra Rudenko, Laura Baseggio, Andrew C Barnes

Abstract read

In one paragraph

Article in FEMS microbiology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Oleksandra RudenkoSchool of the Environment, The University of Queensland, Brisbane QLD 4072, Australia.ORCID 0000-0002-0801-4234

Laura BaseggioSchool of the Environment, The University of Queensland, Brisbane QLD 4072, Australia.

Andrew C BarnesSchool of the Environment, The University of Queensland, Brisbane QLD 4072, Australia.

Funding

Fisheries Research and Development Corporation

6 · The paper itself

Abstract

Photobacterium damselae subsp. piscicida (Pdp) is a host-adapted primary pathogen impacting finfish aquaculture worldwide, whose virulence evolution is driven by the mobilome. The pPHDPT3 plasmid encoding a type III secretion system is critical for Pdp virulence but unstable in vitro in European and Japanese isolates. Here we show that a stable ancestral variant is conserved in Australian isolates. The elusive pPHDPT3 variant has undergone gene loss and accumulated a ∼5.5 kb quadruple direct repeat, which could explain plasmid loss via the classic dimer catastrophe scenario. In addition, we hypothesize that a 189-aa serine recombinase encoded within this repeat, and also on pPHDP10 plasmid, may act as a plasmid resolvase, with its frequent loss further exacerbating the dimer catastrophe.

Indexed as

PhotobacteriumPlasmidsType III Secretion SystemsAnimalsAustraliaGenome, BacterialType III Secretion SystemsPhotobacterium damselae subsp. piscicida (Pdp)plasmid dimersplasmid repeatspPHDP10pPHDPT3putative resolvase

Identifiers

PMID42340358

DOI10.1093/femsle/fnag074

PMCPMC13347740

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.