Evidence map›Paper›PMID 42338215›Full record

ArticleMicrobial biotechnology2026

Engineering Pseudomonas protegens as a Targeted Antifungal-Effector Delivery Chassis via Heterologous Type III Secretion System Reconstitution.

Yuyuan Peng, Simei He, Guofan Zhang, Jiatao Yang, Rong Fan, Chen Li, Jiajie Xie, Xinran Long, Linger Qian, Jiayi Wang and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Yuyuan PengHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.ORCID https://orcid.org/0009-0008-7348-0064
Simei HeHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Guofan ZhangHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Jiatao YangHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Rong FanHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Chen LiHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Jiajie XieHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Xinran LongHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Linger QianHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Jiayi WangHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Yanmin HeHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Hui CaiHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Yushan ZhangHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Qiuqian ZhangHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.
Shaopeng PanHunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Sciences, Hunan Normal University, Changsha, China.
Shengbiao HuHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Sciences, Hunan Normal University, Changsha, China.ORCID https://orcid.org/0000-0001-9236-6496

Funding

Hunan Provincial Natural Science Foundation Basic Research Project for Young Students 2026JJ90210National Key Research and Development Program of China 2023YFD2400503National Key Research and Development Program of China 2023YFD2400504Postgraduate Scientific Research Innovation Project of Hunan Province CX20240476Postgraduate Scientific Research Innovation Project of Hunan Province CX20250780
6 · The paper itself

Abstract

This study addresses the core challenge of Fusarium wilt control in agricultural production. We successfully reconstituted a functional heterologous type III secretion system (T3SS) from Photorhabdus luminescens in the biocontrol bacterium Pseudomonas protegens Pf-5, creating an engineered molecular syringe for targeted delivery of antifungal effectors. The system is activated under low-calcium conditions, achieved by cultivation in calcium-limited medium followed by EGTA-mediated chelation of residual Ca

Indexed as

Antifungal AgentsFusariumPseudomonasType III Secretion SystemsBacterial ProteinsCalciumPhotorhabdusPlant DiseasesSolanum lycopersicumAntifungal AgentsBacterial ProteinsCalciumType III Secretion Systemsantifungal effectorbiological controlFusarium wiltprotein deliveryPseudomonas protegens Pf‐5type III secretion system (T3SS)

Identifiers

PMID42338215
PMCPMC13291435

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.