Evidence map›Paper›PMID 42338006›Full record

ArticleJournal of extracellular vesicles2026

Bacterial Extracellular Vesicles (BEVs) Derived from Chryseobacterium Inhibit Dengue Virus Infection by Disrupting Its Structural Integrity.

Yaqi Gao, Lijian Zhang, Tianci Zhang, Qiufeng Yao, Ruifang Gao, Yue Wang, Yunpeng Zhao, Tingting Zhou, Jikuai Chen, Xing Zhang and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Yaqi GaoDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Lijian ZhangDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Tianci ZhangDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Qiufeng YaoDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Ruifang GaoDepartment of Stem Cell and Regeneration Medicine, Translational Medicine Research Center, Naval Medical University, Shanghai, China.
Yue WangDepartment of Stem Cell and Regeneration Medicine, Translational Medicine Research Center, Naval Medical University, Shanghai, China.
Yunpeng ZhaoDepartment of Stem Cell and Regeneration Medicine, Translational Medicine Research Center, Naval Medical University, Shanghai, China.
Tingting ZhouDepartment of Pharmaceutical Analysis, School of Pharmacy, Naval Medical University, Shanghai, China.
Jikuai ChenDepartment of Health Toxicology, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Xing ZhangDepartment of Health Toxicology, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Hao RenDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Yongzhe ZhuDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Ping ZhaoDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Zhongtian QiDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.
Li LuoShanghai Key Laboratory of Bio-energy Crops, Center of Plant Science, School of Life Sciences, Shanghai University, Shanghai, China.
Zhaoling QinDepartment of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine, Naval Medical University, Shanghai, China.ORCID https://orcid.org/0000-0003-4525-391X

Funding

Naval Medical University 2023 Innovation Capability Development FundNaval Medical University 2024 Cultivation Project of Basic Medical Research 2024MS003Shanghai University Interdisciplinary Collaborative Project LH2025035
6 · The paper itself

Abstract

Dengue virus (DENV) infection poses a significant global health threat, and current prevention and treatment strategies are limited by challenges of lacking effective mosquito control measures and antibody-dependent enhancement. This study reports that bacterial extracellular vesicles (BEVs) secreted by a soil bacterium Chryseobacterium aquifrigidense M24 exhibit potent anti-DENV activity by triggering the structural disintegration of DENV particles prior to cellular entry in a dose-dependent manner. Mechanistic investigations revealed that BEVs interact with the viral envelope, inducing premature membrane fusion. This process is characterized by reduced membrane fluidity and irreversible lipid rearrangement, leading to a significant increase in particle density, as shown by iodixanol gradient ultracentrifugation. The proposed 'fusion-triggered structural disruption' is further supported by the induction of aberrant E protein oligomerization and morphological changes observed via transmission electron microscopy. This mechanism is specific to enveloped viruses, as BEVs showed no effect on non-enveloped Enterovirus 71. Crucially, this BEV-mediated inactivation extends to other enveloped viruses, including HCV, WNV and YFV, indicating broad-spectrum potential. Our findings reveal a previously unexplored function of BEVs as virucidal agents, proposing a new 'virus-destructor' strategy that contrasts with conventional fusion inhibitors and offering promising avenues for developing broad-spectrum antiviral drugs.

Indexed as

Antiviral AgentsChryseobacteriumDengueDengue VirusExtracellular VesiclesAnimalsHumansAntiviral Agentsantiviral mechanismbacterial extracellular vesiclesChryseobacteriumdengue virusenveloped virus

Identifiers

PMID42338006
PMCPMC13291215

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.