Evidence map›Paper›PMID 42337626›Full record

ArticleParasites & vectors2026

A descriptive assessment of mitochondrial COI genetic diversity and dengue and chikungunya RNA detection in Aedes aegypti across eight provinces in Thailand.

Padet Siriyasatien, Proawpilart Intayot, Rungfar Boonserm, Rinnara Ampol, Nataya Sutthanont, Nutticha Silakom, Charuai Suwanbamrung, Jonas Schmidt-Chanasit, Atchara Phumee

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Article in Parasites & vectors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

9 authors.

Padet SiriyasatienDepartment of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Proawpilart IntayotPharmaceutical Ingredient and Medical Device Research Division, Research Development and Innovation Department, The Government Pharmaceutical Organization, Bangkok, Thailand.
Rungfar BoonsermDepartment of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Rinnara AmpolDepartment of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Nataya SutthanontDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Nutticha SilakomProgram in Bioinformatics and Computational Biology, College of Interdisciplinary and Integrative Studies, Chulalongkorn University, Bangkok, Thailand.
Charuai SuwanbamrungExcellent Center for Public Health Research: EC for PHR, Walailak University, Nakhon Si Thammarat, Thailand.
Jonas Schmidt-ChanasitBernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
Atchara PhumeeExcellent Center for Public Health Research: EC for PHR, Walailak University, Nakhon Si Thammarat, Thailand. atchara.ph@wu.ac.th.ORCID https://orcid.org/0000-0001-8243-7406

Funding

National Research Council of Thailand (NRCT) Grant No. NRCT5-RSA63001-03
6 · The paper itself

Abstract

backgroundAedes aegypti is the principal vector of several medically important arboviruses, including dengue virus (DENV) and chikungunya virus (CHIKV), both of which remain endemic in Thailand. However, studies on arbovirus surveillance and mitochondrial genetic diversity of Ae. aegypti populations across broad geographic scales remain limited. This study aimed to assess the detection of DENV and CHIKV and characterize the mitochondrial genetic diversity of Ae. aegypti across multiple regions of Thailand.

methodsA total of 303 adult Ae. aegypti mosquitoes collected from eight provinces across five geographic regions of Thailand were screened for DENV and CHIKV RNA. Mitochondrial cytochrome c oxidase subunit I (COI) genetic diversity was assessed using haplotype network analysis and analysis of molecular variance (AMOVA).

resultsCHIKV RNA was detected in 51 mosquitoes (16.8%) from four provinces, with the highest detection rates observed in Prachuap Khiri Khan and Bangkok. DENV RNA was not detected in any sample. COI analysis revealed moderate to high mitochondrial haplotype diversity and geographic variation in haplotype distribution among populations. Both widely shared and province-specific haplotypes were identified. AMOVA indicated that most genetic variation occurred within populations (93.19%), with significant genetic differentiation among populations (ΦST = 0.068, P < 0.001).

conclusionsCHIKV RNA was detected in Ae. aegypti populations from multiple regions of Thailand, whereas DENV RNA was not detected. Mitochondrial COI analysis revealed substantial haplotype diversity and geographic variation among populations. These findings contribute to current knowledge of arbovirus occurrence and mosquito genetic diversity in Thailand and provide a foundation for future vector surveillance studies.

Indexed as

AedesChikungunya virusDengue VirusElectron Transport Complex IVGenetic VariationMosquito VectorsRNA, ViralAnimalsDengueHaplotypesMitochondriaPhylogenyThailandElectron Transport Complex IVRNA, ViralAedes aegyptiChikungunya virusCytochrome c oxidase subunit IDengue virusGenetic diversityThailand

Identifiers

PMID42337626
PMCPMC13548707

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