Association of IBS-associated E. coli strains with epithelial inflammatory responses and tight junction dysregulation in Caco-2 cells.

Zeinab Merzaei, Hossein Kazemian, Zahra Bamzadeh, Mansoureh Paknejadi, Ebrahim Kouhsari

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Article in BMC research notes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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5 authors.

Zeinab MerzaeiDepartment of Microbiology, ShQ.C., Islamic Azad University, Shahr-e Qods, Iran.

Hossein KazemianDepartment of Microbiology, ShQ.C., Islamic Azad University, Shahr-e Qods, Iran. h.kazemian@outlook.com.

Zahra BamzadehDepartment of Microbiology, ShQ.C., Islamic Azad University, Shahr-e Qods, Iran.

Mansoureh PaknejadiDepartment of Microbiology, ShQ.C., Islamic Azad University, Shahr-e Qods, Iran.

Ebrahim KouhsariLaboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveIrritable bowel syndrome (IBS) presents with symptoms of pain, abdominal discomfort, and altered bowel habits. Changes in the intestinal microbiota, including an increase in Enterobacteriaceae like E. coli, have been associated with IBS. The aim of this study was to evaluate the effects of IBS-associated E. coli strains on epithelial inflammatory responses, tight junction-related gene expression, and Toll-like receptor regulation in Caco-2 cells.

resultsResults showed that IBS-associated E. coli strains exhibited significantly higher invasion activity and increased production of IL-8 and TNF-α compared to H-E. coli. Furthermore, IBS-associated E. coli strains upregulated TLR-4 and TLR-5 expression and reduced the expression of tight junction proteins.

Indexed as

Escherichia coliInflammationIntestinal MucosaIrritable Bowel SyndromeTight JunctionsCaco-2 CellsHumansInterleukin-8Intestinal Barrier FunctionToll-Like Receptor 4Toll-Like Receptor 5Tumor Necrosis Factor-alphaInterleukin-8TLR4 protein, humanTLR5 protein, humanToll-Like Receptor 4Toll-Like Receptor 5Tumor Necrosis Factor-alphaCaco-2 cellsCytokinesCytoskeletal proteinsEscherichia coliIrritable bowel syndrome

Identifiers

PMID42337604

DOI10.1186/s13104-026-07924-1

PMCPMC13386935

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.