ArticleJournal of nanobiotechnology2026
Metal-driven nanoassembly of hexahistidine-tagged melittin enables superior phytopathogen biofilm degradation with attenuated toxicity.
Article in Journal of nanobiotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
Bacterial biofilms formed by phytopathogens confer formidable resistance to chemical pesticides, underscoring an urgent need for innovative antimicrobial solutions. Antimicrobial peptides (AMPs), exemplified by the potent bee venom derivative melittin, offer a promising alternative owing to their broad-spectrum activity and intrinsic biofilm-disrupting capacity. However, the agricultural application of melittin is severely hindered by rapid environmental degradation, susceptibility to enzymatic degradation, and non-selective cytotoxicity. Here, we report a metal-coordination-driven nanoassembly strategy to enhance the stability and efficacy of melittin. Engineering an N-terminal hexahistidine tag enabled a one-step assembly of melittin into uniform nanoparticles (NanoMel) via Zn²⁺ coordination. This nanoformulation improved the antibacterial potency, lowering the half-maximal effective concentration (EC₅₀) values against Xanthomonas oryzae pv. oryzae (Xoo), Xanthomonas oryzae pv. oryzicola (Xoc) to 3.795 µg/mL and 3.202 µg/mL, representing a 1.59- and 1.38-fold enhancement over its linear counterpart. Furthermore, NanoMel demonstrated superior biofilm eradication, degrading 86.9% of mature Xoo biofilms at 24 µg/mL, significantly outperforming the free peptide. In planta assays revealed that NanoMel provided 68.2% curative and 65.9% protective efficacy against rice bacterial leaf blight at 200 µg/mL, surpassing the commercial bactericide thiodiazole-copper 20% suspension concentrate (TC-20% SC). Furthermore, the nanoassemblies effectively attenuated the inherent toxicity of melittin, as evidenced by significantly improved safety profiles in the zebrafish model. Collectively, these findings establish the metal-coordination-driven nanoassembly as a platform for constructing effective and eco-friendly AMP-based bionanobactericides, demonstrating a potent and practical strategy for sustainable plant protection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.