Evidence map›Paper›PMID 42337177›Full record

ArticleParasitology research2026

Molecular detection of Toxoplasma gondii in marine fish from Tunisia: First report in the Southern Mediterranean Sea.

Ghozzi Khemissa, Ibtissem Lahmar, Anna Maria Fausta Marino, Alessandra Aparo, Rafika Challouf, Rym Ben Dhiab, Raja Chaâbane Banaoues, Najoua Haouas

Abstract read
In one paragraph

Article in Parasitology research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ghozzi KhemissaNational Institute of Marine Sciences and Technologies, Marine Biodiversity Laboratory - LR16INSTM02, University of Carthage, 28 Street of March 02, 1934, Salammbô, 2025, Tunisia. khemissa.ghozzi@instm.rnrt.tn.ORCID http://orcid.org/0000-0002-6143-8212
Ibtissem LahmarFaculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology LP3M (code LR12ES08), Department of Clinical Biology B, Monastir University, Monastir, 5000, Tunisia.
Anna Maria Fausta MarinoNational Reference Centre for Toxoplasmosis, Istituto Zooprofilattico Sperimentale della Sicilia, Catania, Italy.
Alessandra AparoNational Reference Centre for Toxoplasmosis, Istituto Zooprofilattico Sperimentale della Sicilia, Catania, Italy.
Rafika ChalloufNational Institute of Marine Sciences and Technologies, Marine Biodiversity Laboratory - LR16INSTM02, University of Carthage, 28 Street of March 02, 1934, Salammbô, 2025, Tunisia.
Rym Ben DhiabNational Institute of Marine Sciences and Technologies, Marine Biodiversity Laboratory - LR16INSTM02, University of Carthage, 28 Street of March 02, 1934, Salammbô, 2025, Tunisia.
Raja Chaâbane BanaouesFaculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology LP3M (code LR12ES08), Department of Clinical Biology B, Monastir University, Monastir, 5000, Tunisia.
Najoua HaouasFaculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology LP3M (code LR12ES08), Department of Clinical Biology B, Monastir University, Monastir, 5000, Tunisia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Toxoplasmosis, caused by Toxoplasma gondii (T. gondii), is a ubiquitous zoonotic parasitic disease increasingly reported in marine ecosystems, raising concerns about seafood contamination and potential human exposure. Consumption of raw or undercooked fish may therefore represent a potential risk to consumers. This study aimed to investigate the presence of T. gondii DNA in marine fish from the Tunisian coast and to assess their potential role as indicators of environmental contamination. A total of 559 specimens, representing 32 species, were collected and grouped into 100 pooled samples by species. Tissue samples (gills, skin/muscle, and intestine) were analyzed using real-time PCR. Overall, 16% (16/100) of pooled samples were tested positive for T. gondii, involving 13 fish species. Positive detections were observed in both wild and farmed fish, including caged Sparus aurata. Gill tissues showed the highest frequency of detection (9/16), followed by lower detection rates in skin/muscle (4/16) and intestinal (3/16) samples. Demersal species showed the highest number of positive pools, followed by pelagic and benthopelagic specimens. However, Carnivorous fish showed higher detection rates (36%) compared to omnivorous (31.6%) and herbivorous species (14.3%). This survey provides the first molecular evidence of T. gondii in marine fish from Tunisia and highlights the potential role of certain species as indicators of environmental contamination and passive carriers of this parasite within marine ecosystems.

Indexed as

Fish DiseasesFishesToxoplasmaToxoplasmosis, AnimalAnimalsDNA, ProtozoanIntestinesMediterranean SeaReal-Time Polymerase Chain ReactionSkinTunisiaDNA, ProtozoanFeeding habit, transmissionFishHabitatT. gondii

Identifiers

PMID42337177
PMCPMC13547175

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.