ArticleScientific data2026
A telomere-to-telomere gapfree genome assembly of Triplophysa stoliczkae (Tibetan stone loach, family Nemacheilidae).
Article in Scientific data, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The Tibetan loach genus Triplophysa exhibits distinct adaptive traits to cope with the unique environmental pressures of the Qinghai-Xizang Plateau. To decipher the molecular mechanisms underlying adaptation to extreme plateau environments, this study constructed the first gapless telomere-to-telomere (T2T) genome of Triplophysa stoliczkae, by integrating PacBio HiFi, Oxford Nanopore ultra-long read, and Hi-C sequencing technologies. The assembly spans 659.99 Mb with a contig N50 of 25.8 Mb and has been fully scaffolded into 25 chromosome-scale sequences, with all centromeres and telomeres completely resolved. Our annotation identified 251.33 Mb (38.08%) of repetitive sequences and 29,030 protein-coding genes. Comparative genomic analyses with related species confirmed the high completeness, contiguity, and accuracy of this assembly. Further supporting the assembly quality, we calculated a quality value (QV) of 41.64 and a BUSCO completeness score of 98.50%. This chromosome-level T2T genome serves as a high-value genomic resource for the genus Triplophysa and provides a critical reference for future studies aimed at elucidating the genetic basis of high-altitude adaptation in plateau fishes.
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