Evidence map›Paper›PMID 42336286›Full record

ArticleThe Journal of biological chemistry2026

Rapid reduction in global chromatin loop size after acute STAG2 reconstitution in human cancer cells.

Tianyi Yang, Jung-Sik Kim, Clara Mellows, Wanying Xu, Alvin Ya, Lisa Sadzewicz, Luke Tallon, Jann Sarkaria, Fulai Jin, Todd Waldman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Tianyi YangThe Biomedical Sciences Training Program, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA; Department of Genetics and Genome Sciences, Case Comprehensive Cancer Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA.
Jung-Sik KimDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University School of Medicine, Washington, District of Columbia, USA.
Clara MellowsDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University School of Medicine, Washington, District of Columbia, USA.
Wanying XuThe Biomedical Sciences Training Program, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA; Department of Genetics and Genome Sciences, Case Comprehensive Cancer Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA.
Alvin YaDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University School of Medicine, Washington, District of Columbia, USA; Tumor Biology Training Program, Georgetown University School of Medicine, Washington, District of Columbia, USA.
Lisa SadzewiczInstitute for Genome Sciences, University of Maryland, Baltimore, Maryland, USA.
Luke TallonInstitute for Genome Sciences, University of Maryland, Baltimore, Maryland, USA.
Jann SarkariaDepartment of Radiation Oncology, Mayo Clinic, Rochester, Minnesota, USA.
Fulai JinDepartment of Genetics and Genome Sciences, Case Comprehensive Cancer Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA. Electronic address: fxj45@case.edu.
Todd WaldmanDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University School of Medicine, Washington, District of Columbia, USA. Electronic address: waldmant@georgetown.edu.

Funding

Robust mapping of chromatin loops from sparse or single cell Hi-C data with DeepLoopR01HG009658 · NHGRI · CASE WESTERN RESERVE UNIVERSITY · PI Fulai Jin · 2017 to 2026
$5.1M
STAG2 mutations and 3D genome organization in glioblastoma multiformeR01CA267872 · NCI · GEORGETOWN UNIVERSITY · PI Fulai Jin, TODD A WALDMAN · 2022 to 2026
$2.7M
Mapping heritable chromatin loop variants with allele-specific Hi-C analysisR01HG012384 · NHGRI · CASE WESTERN RESERVE UNIVERSITY · PI Alan D Attie, Fulai Jin · 2023 to 2026
$2.6M
Understanding the variation of induced β-cell differentiation.R01DK131437 · NIDDK · CASE WESTERN RESERVE UNIVERSITY · PI JIN, FULAI, LI, YAN · 2022 to 2025
$2.4M
NCI NIH HHS R01 CA267872NHGRI NIH HHS R01 HG009658NHGRI NIH HHS R01 HG012384NIDDK NIH HHS R01 DK131437
6 · The paper itself

Abstract

Truncating mutations in the tumor suppressor STAG2, which encodes a component of the cohesin complex, are prevalent across diverse human cancers. Here, we report that acute reconstitution of physiological STAG2 levels in STAG2-mutant human glioblastoma multiforme cancer cells triggers a rapid reduction in the size of chromatin loops genome-wide. Despite this global change in chromatin loop size, early transcriptional responses to STAG2 restoration are limited to a small number of genes, most of which are induced by STAG2 reconstitution. Notably, the growth-suppressor EFEMP1 (Fibulin-3), a secreted glycoprotein that functions as an extracellular matrix-associated inhibitor of glioblastoma growth and invasion, was the only gene consistently induced across all experimental models. The most robust and conserved STAG2-induced genes all reside within intense chromatin loops whose anchors and overall intensity did not appear to change in response to STAG2 reconstitution. These findings suggest that inactivating mutations of STAG2 promote neoplastic transformation by alleviating a restriction on chromatin loop size, allowing for an expanded range of chromatin interactions that disrupts the maintenance of a tumor-suppressive transcriptome.

Indexed as

Antigens, NuclearChromatinGlioblastomaCell Line, TumorCohesinsGene Expression Regulation, NeoplasticHumansMutationAntigens, NuclearChromatinCohesinsSTAG2 protein, human

Identifiers

PMID42336286
PMCPMC13393664

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.