ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2026
Development of a digital loop-mediated isothermal amplification using a digital polymerase chain reaction device.
Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This study established a robust digital loop-mediated isothermal amplification (dLAMP) workflow using the QIAcuity nanoplate-based dPCR system to overcome the limitations of traditional isothermal reactions and low partition counts. By optimizing reactions targeting the mitochondrial CO1 region of Limnoperna fortunei with diluted EvaGreen Master Mix, the method achieved high-throughput absolute quantification across a wide dynamic range without requiring standard curves. Results demonstrated that while conventional turbidimetric LAMP showed moderate variability accompanied by occasional delayed amplification at low concentrations, dLAMP maintained stable and clear detection at 1.52 × 10
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