Evidence map›Paper›PMID 42334512›Full record

ArticleDiscover nano2026

Green synthesis of zinc oxide nanoparticles using Cassia absus leaf extract and evaluation of their biofertilizer potential and in vitro antibacterial activity against phytopathogens.

Hassan Iqbal, Zia Ur Rehman, Muhammad Jamil, Nigar Ahmad, Yehia Hazzazi, Nasir Assad, Rahila Malik, Musawir Ahmad, Mohammad Shariq, Ahmed Vandy and 1 more

Abstract read
In one paragraph

Article in Discover nano, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Hassan IqbalDepartment of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, 26000, Khyber Pakhtunkhwa, Pakistan.
Zia Ur RehmanDepartment of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, 26000, Khyber Pakhtunkhwa, Pakistan. z.rehman@kust.edu.pk.
Muhammad JamilDepartment of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, 26000, Khyber Pakhtunkhwa, Pakistan.
Nigar AhmadDepartment of Chemistry, Hazara University Mansehra, Mansehra, Pakistan.
Yehia HazzaziDepartment of Biology, College of Science, Jazan University, P.O. Box 114, Jazan, 45142, Saudi Arabia.
Nasir AssadInstitute of Chemistry, University of Sargodha, Sargodha, 40100, Pakistan.
Rahila MalikInstitute of Chemistry, University of Sargodha, Sargodha, 40100, Pakistan.
Musawir AhmadDepartment of Zoology, Kohat University of Science and Technology, Kohat, 26000, Khyber Pakhtunkhwa, Pakistan.
Mohammad ShariqDepartment of Physical Sciences, Physics Division, College of Science, Jazan University, Jazan, 45142, Saudi Arabia. mohammadshariq@jazanu.edu.sa.
Ahmed VandyFaculty of Pharmaceutical Sciences, College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone. ahmedvandy@gmail.com.
Mukul SharmaEnvironment and Nature Research Centre, Jazan University, P.O. Box 114, Jazan, 45142, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conventional agriculture on chemical fertilizers and pesticides has serious environmental and health implications. This necessitates the development of more sustainable and eco-friendly alternatives. This study aimed to synthesize zinc oxide nanoparticles (ZnONPs) using Cassia absus leaf extract via a green synthesis approach and evaluate their dual role as biofertilizers and biopesticides in sustainable agriculture. The biosynthesized  ZnONPs were characterized using UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX), dynamic light scattering (DLS), and zeta potential analysis. A characteristic absorption peak at 355 nm confirmed nanoparticle (NP) formation, whereas XRD analysis revealed a hexagonal wurtzite structure with a crystallite size of 45-55 nm. SEM analysis showed spherical to quasi-spherical NPs with an average size of 75 nm, whereas DLS indicated a hydrodynamic diameter of 83.5 nm and a zeta potential of + 48.7 mV, confirming excellent colloidal stability. The in vitro antibacterial activity of ZnONPs was evaluated against the phytopathogens Ralstonia solanacearum, Xanthomonas euvesicatoria, and Clavibacter michiganensis subsp. michiganensis. Significant inhibition zones of 20 mm, 18 mm, and 27.5 mm, respectively, were observed, with minimum inhibitory concentrations (MICs) of 30, 40, and 20 µg/mL. In pot experiments using Capsicum annuum, ZnONPs significantly enhanced plant growth parameters, including plant height, leaf length, and leaf width, with up to a 37% improvement observed at moderate concentrations (5 µg/10 mL). These results demonstrate that ZnONPs offer an effective dual strategy for enhancing plant growth and controlling bacterial pathogens, making them promising candidates for sustainable agricultural applications.

Indexed as

BiofertilizerBiopesticidesCassia absusGreen synthesisZinc oxide nanoparticles

Identifiers

PMID42334512
PMCPMC13291277

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.