Observational studyInternational endodontic journal2026
Distinct Microbiota and Functional Pathway Profiles Define Success and Failure in Regenerative Endodontic Treatment.
Observational study in International endodontic journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
aimTo characterize the intracanal microbiota and identify key microbial taxa and functional pathways associated with regenerative endodontic treatment (RET) outcomes. METHODOLOGY: In this observational cohort study, 196 samples were collected at five time points from external tooth surfaces (T1, T4) and root canals (T2, T3, T5). The V3-V4 hypervariable region of 16S rRNA gene was amplified and sequenced on the Illumina MiSeq platform. Sequence data were pre-processed using QIIME2 and DADA2 to generate amplicon sequence variants (ASVs), classified against the eHOMD database. Microbial diversity, differential abundance, and Receiver Operating Characteristic (ROC) analyses were performed using R statistical software. Functional profiles were predicted using PICRUSt2.
resultsRET induced dynamic shifts in the root canal microbiome, with initially predominant phyla including Actinomycetota, Bacillota, Bacteroidota, Fusobacteriota and Pseudomonadota. Cases with treatment failure consistently showed a higher relative abundance of Fusobacteriota than successful cases. Alpha diversity varied across time points, with outcome-specific differences observed only at T3; beta diversity differed significantly by time point but not by treatment outcome. Differential abundance analysis at T5 revealed enrichment of Rothia dentocariosa and Leptothrix sp. in successful cases, whereas failed cases were enriched in Fusobacterium polymorphum, Streptococcus sanguinis, Campylobacter showae, Lachnoanaerobaculum sp. and Selenomonas sp. These taxa demonstrated moderate diagnostic potential, with area under the curve (AUC) values of 0.70-0.82. Persistence analysis showed that F. polymorphum, C. showae and Selenomonas sp. exhibited greater persistence in failed cases across treatment time points. Predicted functional profiling indicated distinct metabolic potentials between successful and failed treatment groups.
conclusionsRET alters the intracanal microbiome, with taxa identified following intracanal dressing exhibiting moderate predictive potential as biomarkers for treatment outcome, thereby contributing to early prognosis and informing RET strategies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.