Evidence map›Paper›PMID 42333778›Full record

ArticleTransboundary and emerging diseases2026

Development of a Blocking ELISA for the Serological Diagnosis of Getah Virus.

Shiyi Shen, Zhengxin Yang, Yumei Sun, Weidong Yan, Mengjia Zhang, Hailong Ma, Wenjuan Du, Ahmed H Ghonaim, Anan Jongkaewwattana, Suphot Wattanaphansak and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Shiyi ShenNational Key Laboratory of Agricultural Microbiology and Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China, hzau.edu.cn.
Zhengxin YangNational Key Laboratory of Agricultural Microbiology and Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China, hzau.edu.cn.ORCID https://orcid.org/0000-0001-5832-9253
Yumei SunNational Key Laboratory of Agricultural Microbiology and Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China, hzau.edu.cn.
Weidong YanWuhan Keqian Biology Co. Ltd., Wuhan, 430072, China.
Mengjia ZhangNational Key Laboratory of Agricultural Microbiology and Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China, hzau.edu.cn.
Hailong MaNational Key Laboratory of Agricultural Microbiology and Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China, hzau.edu.cn.
Wenjuan DuCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China, henau.edu.cn.
Ahmed H GhonaimNational Key Laboratory of Agricultural Microbiology and Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China, hzau.edu.cn.
Anan JongkaewwattanaNational Center for Genetic Engineering and Biotechnology, Pathum Thani, 12120, Thailand, biotec.or.th.
Suphot WattanaphansakDepartment of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Pathum Wan, 10330, Bangkok, Thailand, chula.ac.th.
Qigai HeNational Key Laboratory of Agricultural Microbiology and Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China, hzau.edu.cn.ORCID https://orcid.org/0000-0001-7005-7473
Wentao LiNational Key Laboratory of Agricultural Microbiology and Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China, hzau.edu.cn.ORCID https://orcid.org/0000-0002-7114-762X

Funding

Earmarked Fund for China Agriculture Research System CARS-35National Key Research and Development Program of China 2025YFC3507700Yingzi Tech & Huazhong Agricultural University Intelligent Research Institute of Food Health IRIFH202209
6 · The paper itself

Abstract

Getah virus (GETV) is an emerging arbovirus that poses a growing threat to the swine industry because of its ability to infect pigs and other animals. However, the lack of an effective porcine GETV vaccine and commercially available diagnostic tools has hindered control efforts. In the study, we expressed the recombinant GETV E2 protein in a mammalian cell system to preserve its native conformation and antigenicity and generated monoclonal antibodies (mAbs) by immunizing mice with this protein. Using the GETV E2 protein and horseradish peroxidase (HRP)-labeled mAbs, we successfully established a rapid and specific blocking ELISA for the serological detection of GETV. The assay was comprehensively evaluated for specificity, reproducibility, and consistency with the reference immunofluorescence assay (IFA), demonstrating reliable diagnostic performance. In a clinical evaluation involving 462 samples, the assay showed a positive rate of 12.121%, confirming its practical applicability. This novel blocking ELISA provides a valuable tool for vaccine development and epidemiological investigations, contributing to more effective strategies for GETV control.

Indexed as

AlphavirusAlphavirus InfectionsSwine DiseasesAnimalsAntibodies, MonoclonalAntibodies, ViralEnzyme-Linked Immunosorbent AssayMiceMice, Inbred BALB CReproducibility of ResultsSensitivity and SpecificitySwineAntibodies, MonoclonalAntibodies, Viralblocking ELISAdiagnosisE2 proteingetah virusmonoclonal antibody

Identifiers

PMID42333778
PMCPMC13287966

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.