Evidence map›Paper›PMID 42332827›Full record

ArticleVirology journal2026

Evaluation of a portable multiplex real-time pcr assay for the detection of monkeypox virus and non-variola orthopoxviruses.

Shanshan Li, Nabojeet Das, Natalie Miller, Ariel Caudle, Kimberly Wilkins, Theodora Khan, Stefan Pfotenhauer, Amy Saunders, Christina L Hutson, Kodumudi S Venkateswaran and 4 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Shanshan Li *Poxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Nabojeet Das *Poxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Natalie MillerPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Ariel CaudlePoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Kimberly WilkinsPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Theodora KhanPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Stefan PfotenhauerTetracore Inc., Rockville, MD, USA.
Amy SaundersTetracore Inc., Rockville, MD, USA.
Christina L HutsonPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Kodumudi S VenkateswaranTetracore Inc., Rockville, MD, USA.
William M NelsonTetracore Inc., Rockville, MD, USA.
Neeraja VenkateswaranTetracore Inc., Rockville, MD, USA.
Panayampalli S SatheshkumarPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Michael B TownsendPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA. gbu3@cdc.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monkeypox (mpox) is the most significant orthopoxvirus (OPXV) infection in humans since smallpox eradication. The World Health Organization (WHO) has twice declared public health emergency of international concern due to the expansive spread of clade I and II mpox cases, thus prompting public health scientists to develop rapid and effective diagnostic tools. Clinical presentations of monkeypox can overlap with other vesiculopustular diseases, particularly those caused by Varicella-Zoster virus (VZV), complicating accurate diagnosis in decentralized or resource-limited settings. In this study, we performed an in-lab evaluation of a multiplexed real-time PCR on a portable platform (T-COR 8) for the detection of monkeypox virus (MPXV) and non-variola orthopoxvirus (NVAR-OPXV), and differentiation from varicella-zoster virus (VZV). The T-COR 8 platform required minimal technical skill, limited hands-on time, and generated results for 8 samples within 1 h using crude specimens, without the need for nucleic acid extraction. The performance characteristics were investigated using MPXV and VZV clinical sample remainders and a panel of laboratory cultured viruses. Testing of 116 clinical remainder specimens in the MPXV/NVAR-OPXV assay yielded 100% sensitivity, 97.8% specificity, and 99.1% agreement with prior test results. The MPXV/NVAR-OPXV/VZV assay detected and correctly differentiated MPXV and VZV in a limited set of clinical samples (n = 39). The MPXV assay demonstrates specificity for MPXV, except for Akhmeta virus (AKMV), out of the 20 different species/isolates tested, including 15 orthopoxviruses and 4 non-orthopoxviruses, and VZV. These findings support the T-COR 8 platform as a rapid, accurate, and field-deployable diagnostic tool suitable for expanding monkeypox testing capacity.

Indexed as

Molecular Diagnostic TechniquesMonkeypox virusMpox, MonkeypoxMultiplex Polymerase Chain ReactionOrthopoxvirusPoxviridae InfectionsReal-Time Polymerase Chain ReactionAnimalsHumansRapid Diagnostic TestsSensitivity and SpecificityMonkeypox virusMpoxPCRPoint-of-care

Identifiers

PMID42332827
PMCPMC13536642

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.