Evidence map›Paper›PMID 42332751›Full record

ArticleBMC veterinary research2026

Differential regulation of autophagy and metabolic pathways by dietary methionine and lysine deficiency in goats.

Shabbir Ahmed, Jian Wu, Zixin Liu, Aoyu Jiang, Chuanshe Zhou, Zhiliang Tan, Liang Chen, Jinhe Kang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shabbir AhmedState Key Laboratory of Forage Breeding-by-Design and Utilization, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition & Physiology and Metabolism, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, P.R. China.ORCID http://orcid.org/0000-0003-2209-3837
Jian WuState Key Laboratory of Forage Breeding-by-Design and Utilization, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition & Physiology and Metabolism, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, P.R. China.
Zixin LiuState Key Laboratory of Forage Breeding-by-Design and Utilization, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition & Physiology and Metabolism, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, P.R. China.
Aoyu JiangState Key Laboratory of Forage Breeding-by-Design and Utilization, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition & Physiology and Metabolism, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, P.R. China.
Chuanshe ZhouState Key Laboratory of Forage Breeding-by-Design and Utilization, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition & Physiology and Metabolism, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, P.R. China.
Zhiliang TanState Key Laboratory of Forage Breeding-by-Design and Utilization, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition & Physiology and Metabolism, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, P.R. China.
Liang ChenDepartment of Dairy and Poultry Science, Gazipur Agricultural University, Gazipur, 1706, Bangladesh. cl2021@syau.edu.cn.
Jinhe KangState Key Laboratory of Forage Breeding-by-Design and Utilization, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition & Physiology and Metabolism, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, P.R. China. kangjh@isa.ac.cn.ORCID https://orcid.org/0000-0002-9805-1695

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This research aimed to explore the effect and mechanism of dietary methionine and lysine deficiency on autophagy of gastrointestinal tract and liver tissues. Thirty goats were divided into three groups: control (Con), methionine deficiency (MD), and lysine deficiency (LD) groups. The expression levels of autophagy marker proteins LC3 and p62 were analyzed by immunohistochemistry (n = 5), and mRNA expression levels of autophagy-related genes ATF4, FOXO3, Beclin-1, Atg13 and eIF2α were analyzed through qPCR. No significant differences were found for LC3, but optical density for p62 of jejunal mucosa in LD was significantly higher (P < 0.01) than Con and MD. The mRNA expression level of rumen epithelial ATF4 in LD was significantly higher compared to control and MD, but FOXO3 in LD was significantly higher than control (P < 0.01). In the jejunal mucosa, LD had the highest ATF4 mRNA expression level, while Beclin-1 mRNA expression level was lowest (P < 0.01) compared to control. Metabolomics of jejunal mucosa showed that sorbitol and N-acetylglucosamine in MD and DL-Homocystine and eight other metabolites in LD were significantly increased compared to control. Overall, the degradation process of autophagy was blocked in the jejunum when the diet was lacking of lysine in jejunum, and ATF4 was upregulated to maintain the metabolic balance of amino acids in rumen, jejunum and ileum. However, dietary MD primarily affected metabolic pathways rather than autophagy.

Indexed as

AutophagyGoatsLysineMethionineAnimal FeedAnimalsDietJejunumLiverMetabolic Networks and PathwaysRNA, MessengerRumenLysineMethionineRNA, MessengerAutophagyGastrointestinal tractLC3Metabolomicsp62

Identifiers

PMID42332751
PMCPMC13540822

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.