Evidence map›Paper›PMID 42332695›Full record

ArticleBMC biotechnology2026

Improving xylitol production by semi-rational engineering of xylitol dehydrogenase and optimizing cofactor regeneration in Gluconobacter oxydans.

Jun-Hao Su, Jin-Cheng Guo, Lu Liu, Hai-Ling Zhang, Dong Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jun-Hao SuDepartment of Biological Engineering, School of Life Sciences, Yantai University, Yantai, 264005, China.
Jin-Cheng GuoDepartment of Biological Engineering, School of Life Sciences, Yantai University, Yantai, 264005, China.
Lu LiuDepartment of Biological Engineering, School of Life Sciences, Yantai University, Yantai, 264005, China.
Hai-Ling ZhangDepartment of Biological Engineering, School of Life Sciences, Yantai University, Yantai, 264005, China. hlzhang@ytu.edu.cn.
Dong LiuDepartment of Biological Engineering, School of Life Sciences, Yantai University, Yantai, 264005, China. liudong2021@ytu.edu.cn.ORCID https://orcid.org/0000-0002-6967-3348

Funding

Natural Science Foundation of Shandong Province ZR2022QB111
6 · The paper itself

Abstract

backgroundXylitol, a widely used sugar substitute in food and medicine, can be produced through microbial bioconversion using glucose as the primary substrate. In this process, a critical factor limiting xylitol production is the relatively low activity of xylitol dehydrogenase (XDH) during the biotransformation of D-arabitol to xylitol by resting cells of Gluconobacter oxydans.

resultsTo improve the catalytic performance, GoXDH was engineered by site-saturation mutagenesis combined with a high-throughput screening method, and a variant GoXDH

conclusionThe findings suggest that boosting XDH activity through semi-rational engineering markedly improves xylitol productivity in G. oxydans.

Indexed as

D-Xylulose ReductaseGluconobacter oxydansProtein EngineeringXylitolBiotransformationCoenzymesMutagenesis, Site-DirectedSugar AlcoholsarabitolCoenzymesD-Xylulose ReductaseSugar AlcoholsXylitolCoenzyme regeneration systemGluconobacter oxydansSite-saturation mutagenesisXylitolXylitol dehydrogenase

Identifiers

PMID42332695
PMCPMC13540936

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.