Evidence map›Paper›PMID 42332540›Full record

ArticleBMC genomics2026

The genomic resource of Lysinibacillus fusiformis KBD-5, a biocontrol agent with antifungal activity against Botrytis cinerea.

Yepeng Gao, Qifa Zhu, Lianlian Yuan, Yubing Jiao, Yingwen Wang, Zhouyang Pei, Lili Shen, Ying Li, Sideng Shen, Songbai Zhang and 2 more

Abstract read
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In one paragraph

Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yepeng Gao *MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co- construction by Ministry and Province), Yangtze University, Jingzhou, 434025, China.
Qifa Zhu *Anhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China.
Lianlian YuanKey Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Yubing JiaoKey Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Yingwen WangKey Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Zhouyang PeiAnhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China.
Lili ShenKey Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Ying LiKey Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Sideng ShenAnhui Wannan Tobacco Leaf Co., Ltd, Xuancheng, 242000, China.
Songbai ZhangMARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co- construction by Ministry and Province), Yangtze University, Jingzhou, 434025, China.
Jinguang YangKey Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Binna LvKey Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, State Tobacco Monopoly Administration (China National Tobacco Corporation), Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China. lvbinna@caas.cn.

Funding

National Natural Science Foundation of China 32502575Shandong Provincial Natural Science Foundation ZR2025QC247The Agricultural Science and Technology Innovation Program ASTIP-TRIC-QH-2025B02Tobacco Green Pest Control Project of Anhui Wannan Tobacco Leaf Co., Ltd. WNYY-0563-202502013
6 · The paper itself

Abstract

Lysinibacillus fusiformis strain KBD-5, previously known for its antiviral activity against Tobacco mosaic virus, was investigated for its biocontrol potential against the fungal pathogen Botrytis cinerea. In plate assays, conducted with three independent biological replicates and incubated at 28 °C for 5 days, KBD-5 significantly inhibited the mycelial growth of B. cinerea by 76.42%. Whole-genome sequencing revealed a 4.69 Mb genome with a GC content of 37.28%, encoding 4719 proteins. Bioinformatics analysis identified genes involved in antimicrobial functions, including 195 carbohydrate-active enzymes (potentially aiding in fungal cell wall degradation) and 8 gene clusters for secondary metabolite synthesis (e.g., T3PKS with 30% similarity to bacillibactin biosynthetic clusters and NRPS), indicating the production of antifungal metabolites like bacillibactin-like polyketides. The strain also showed a high safety profile with no significant virulence or drug resistance risks. These findings indicate that genomic analysis of KBD-5 reveals the potential for multiple biocontrol mechanisms, supporting its potential development as a biocontrol agent. The draft genome sequence is available under NCBI accession PRJNA1335659.

Indexed as

Antifungal AgentsBacillaceaeBiological Control AgentsBotrytisGenome, BacterialGenomicsMultigene FamilyWhole Genome SequencingAntifungal AgentsBiological Control AgentsBiological controlCarbohydrate-active enzymesLysinibacillus fusiformisSecondary metabolitesWhole-genome sequencing

Identifiers

PMID42332540

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.