Evidence map›Paper›PMID 42332067›Full record

ArticleEMBO reports2026

The mechanisms of DEPDC7-mediated actin cap establishment and meiosis progression in oocytes.

Jian Mu, Siyuan Xie, Lin Zhao, Yang Zeng, Hao Gu, Zhiqi Pan, Ran Yu, Zhihua Zhang, Tianyu Wu, Weijie Wang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jian Mu *Institute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China.
Siyuan Xie *Institute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China.
Lin Zhao *Department of Bioengineering, University of Pennsylvania, Philadelphia, 19104, PA, USA.
Yang ZengCenter for Reproductive Medicine, Department of Gynecology & Obstetrics, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China.
Hao GuInstitute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China.
Zhiqi PanInstitute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China.
Ran YuInstitute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China.
Zhihua ZhangInstitute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China.
Tianyu WuInstitute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China.ORCID 0000-0002-4295-7172
Weijie WangInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200030, China.ORCID 0000-0002-5717-1818
Lei WangInstitute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China. wangleiwanglei@fudan.edu.cn.ORCID 0000-0002-3400-0434
Qing SangInstitute of Pediatrics, Children's Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, Fudan University, Shanghai, 200032, China. sangqing@fudan.edu.cn.ORCID 0000-0002-9895-1394

Funding

Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China No. JYB2025XDXM508Fund of Fudan University and Cao'ejiang Basic Research 24FCB01MOST | National Key Research and Development Program of China (NKPs) 2024YFC2706600MOST | National Natural Science Foundation of China (NSFC) 32130029MOST | National Natural Science Foundation of China (NSFC) 82288102MOST | National Natural Science Foundation of China (NSFC) 82325021MOST | National Natural Science Foundation of China (NSFC) 82371663
6 · The paper itself

Abstract

Asymmetric division is crucial for oocyte and embryo development. During meiosis I, the spindle migrates to the subcortex, and F-actin accumulates to form the actin cap, which supports polar body extrusion. While the CDC42-NWASP-ARP2/3 pathway, regulated by Ran-GTP, is known to promote actin cap formation, how Ran-GTP controls cortical polarization and the precise function of the actin cap remain unclear. Here, we identify Depdc7 as a novel actin cap-localized protein essential for actin cap formation and first polar body extrusion. DEPDC7 is recruited to the oocyte membrane by Ran-GTP and acts as a scaffold to promote cortical actin assembly. Strikingly, inhibiting CDK1 activity partially rescues the extrusion defect in Depdc7-depleted oocytes. These findings indicate that both CDK1 activity and actin cap formation are essential for polar body extrusion and function synergistically. Our study refines the molecular mechanism of actin cap assembly and highlights DEPDC7 as a key regulator of oocyte maturation and female fertility.

Indexed as

ActinsMeiosisOocytesXenopus ProteinsAnimalsCDC2 Protein KinaseFemalePolar BodiesActinsCDC2 Protein KinaseXenopus Proteins

Identifiers

PMID42332067
PMCPMC13457618

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.