Evidence map›Paper›PMID 42331911›Full record

ArticleScientific reports2026

A combined ex vivo fundus imaging-histology protocol for clinicopathological validation of human donor eyes with limited medical history.

Sarrvesa H Vijay Singh, Waiwai Yeap, Lay Khoon Too, Alexandra Allende, Geoffrey Hall, Alex Hunyor, Michele C Madigan, Rohan Merani, Juan Ortiz, Michael Rodriguez and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Sarrvesa H Vijay Singh *The University of Notre Dame, Darlinghurst, NSW, Australia.
Waiwai Yeap *Medicine & Health, The University of New South Wales, Sydney, NSW, Australia.
Lay Khoon TooSave Sight Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Alexandra AllendeDouglass Hanly Moir Pathology, Macquarie Park, NSW, Australia.
Geoffrey HallDouglass Hanly Moir Pathology, Macquarie Park, NSW, Australia.
Alex HunyorSave Sight Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Michele C MadiganMedicine & Health, The University of New South Wales, Sydney, NSW, Australia.
Rohan MeraniDiscipline of Clinical Ophthalmology and Eye Health, Concord Clinical School, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Juan OrtizDouglass Hanly Moir Pathology, Macquarie Park, NSW, Australia.
Michael RodriguezDouglass Hanly Moir Pathology, Macquarie Park, NSW, Australia.
James WongSave Sight Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Alessandro InvernizziDepartment of Biomedical and Clinical Science, Università degli Studi di Milano, Milan, Lombardy, Italy.
Murray KillingsworthMedicine & Health, The University of New South Wales, Sydney, NSW, Australia.
Victoria PyeSave Sight Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Hemal MehtaThe University of Notre Dame, Darlinghurst, NSW, Australia.
Constantinos PetsoglouSave Sight Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Svetlana CherepanoffThe University of Notre Dame, Darlinghurst, NSW, Australia. s.cherepanoff@unsw.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated a combined ex vivo fundus imaging-histology protocol to improve accuracy of diagnosis in human donor eyes with limited medical history. Fifty-one formalin-fixed eyes from 32 donors underwent a standardised workflow comprising ex vivo fundus photography, selective spectral-domain optical coherence tomography (SD-OCT), and comprehensive histological examination. Medical/surgical retinal ophthalmologists independently reviewed imaging, while ophthalmic pathologists performed masked histological assessment. Diagnostic metrics were calculated using histology as the reference standard. Ex vivo imaging identified definitive pathology in 10 of 51 eyes (19.6%) and possible abnormalities in 4 eyes (7.8%). Histological analysis, however, revealed definitive pathology in 20 eyes (39.2%), detecting a broader range of diseases, including early age-related macular degeneration, hypertensive vasculopathy, and a rare choroidal tumour. Concordance was observed in 27 eyes (52.9%), mainly where no pathology was present. Significant discordance occurred in 24 eyes (47.1%), comprising 13 false negatives and 11 false positives (including misclassified pathology). Consequently, the sensitivity and specificity of ex vivo imaging for detecting pathology were 18.8% and 68.6%, respectively. While ex vivo imaging is a practical screening tool, imaging alone may miss or misclassify pathology due to postmortem artefacts and a lack of validated interpretive criteria. A combined imaging-histology approach validates the limited tissue available from eye-bank programs and maximises research value.

Indexed as

EyeFundus OculiTissue DonorsAdultAgedAged, 80 and overFemaleHumansMaleMiddle AgedSensitivity and SpecificityTomography, Optical CoherenceClinicopathological correlationEx vivo imagingEx vivo validationOphthalmic pathologyPostmortem eyes

Identifiers

PMID42331911
PMCPMC13572494

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.