Evidence map›Paper›PMID 42331894›Full record

ArticleScientific reports2026

Biofilm formation and antimicrobial resistance of Pseudomonas aeruginosa in cheese production systems.

Noura K Ahmed, Dalia Hamza, Shimaa S Awaad, Ayah B Abdel-Salam, Said S Sallam

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Noura K AhmedDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza Square, P.O Box 12211, Giza, Egypt. nourakhalil@cu.edu.eg.ORCID 0009-0007-6005-0455
Dalia HamzaDepartment of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Giza Square, P.O Box 12211, Giza, Egypt.ORCID 0000-0001-7579-5432
Shimaa S AwaadDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza Square, P.O Box 12211, Giza, Egypt.ORCID 0000-0001-9394-2061
Ayah B Abdel-SalamDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza Square, P.O Box 12211, Giza, Egypt.ORCID 0000-0002-1998-6469
Said S SallamDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza Square, P.O Box 12211, Giza, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The emergence of drug-resistant Pseudomonas aeruginosa is an increasing global concern affecting human and animal health, food production systems, and environmental safety. This study investigated its occurrence, antimicrobial resistance, and biofilm-forming ability using phenotypic assays, and identify the biofilm-associated genetic markers in 120 cheese samples including 30 samples each of (Kariesh, Tallaga, Processed, and Romy) collected from various retail sources in Cairo and Giza, Egypt. Pseudomonas aeruginosa isolates were identified using both biochemical tests and molecular confirmation using 16 S rRNA gene. Antimicrobial susceptibility was evaluated using the disk diffusion method. Biofilm formation was assessed phenotypically through the microtiter plate and tube assays, while the biofilm-associated genes pelA and pslA were detected using PCR. Pseudomonas aeruginosa was detected in 18 samples (15%), and identification was confirmed through biochemical and molecular methods. Antimicrobial susceptibility testing revealed that 55% of isolates were extensively drug-resistant, while 45% exhibited multidrug resistance, with MAR indices ≥ 0.2 and an average MAR index of 0.68, indicating exposure to high-risk environments with frequent antibiotic use. Phenotypic assays showed strong biofilm-forming capabilities among isolates, with 100% positive by microtiter plate and 95% by tube method. Molecular screening further confirmed the prevalence of biofilm-associated genes, detecting pelA in 72% and pslA in 61% of isolates. Overall, Pseudomonas aeruginosa isolated from cheese samples exhibited substantial antimicrobial resistance and robust biofilm-forming ability, posing significant concerns to cheese quality and consumer health. These findings highlight the urgent need for continuous surveillance, improved dairy hygiene, effective sanitation, responsible antimicrobial practices, and alternative control strategies within the dairy sector to reduce potential public health hazards.

Indexed as

BiofilmsCheeseDrug Resistance, BacterialPseudomonas aeruginosaAnimalsAnti-Bacterial AgentsDrug Resistance, Multiple, BacterialFood MicrobiologyMicrobial Sensitivity TestsAnti-Bacterial AgentsBiofilmCheeseDairy contaminationFood safetyMultidrug resistanceMultiple antibiotic resistancePseudomonas aeruginosa

Identifiers

PMID42331894
PMCPMC13287804

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.