Evidence map›Paper›PMID 42331843›Full record

ArticleNature communications2026

A sensitive orange fluorescent calcium ion indicator for imaging neural activity.

Abhi Aggarwal, Heather A Baker, Céline D Dürst, I-Wen Chen, Pablo de Chambrier, Carrie Stine, Julia Marie Gonzales, Jonathan S Marvin, Milène Vandal, Torgny Lundberg and 20 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

30 authors.

Abhi AggarwalCumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Heather A BakerUniversity of Alberta, Department of Chemistry, Edmonton, AB, Canada.ORCID http://orcid.org/0000-0002-3006-3008
Céline D DürstDepartment of Basic Neurosciences, and Neurocenter, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
I-Wen ChenDepartment of Basic Neurosciences, and Neurocenter, Faculty of Medicine, University of Geneva, Geneva, Switzerland.ORCID http://orcid.org/0000-0003-0598-7230
Pablo de ChambrierDepartment of Basic Neurosciences, and Neurocenter, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Carrie StineAllen Institute, Neural Dynamics, Seattle, WA, USA.ORCID http://orcid.org/0000-0001-8171-3464
Julia Marie GonzalesCumming School of Medicine, University of Calgary, Calgary, AB, Canada.ORCID http://orcid.org/0009-0008-9541-9766
Jonathan S MarvinJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID http://orcid.org/0000-0003-2294-4515
Milène VandalCumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Torgny LundbergCumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Cherie A Brown-PantonCumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Kenryo SakoiDepartment of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Japan.
Ronak PatelJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Ching-Yao WangInstitute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
Frank VisserHotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada.
Yannick FouadCumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Smrithi SunilAllen Institute, Neural Dynamics, Seattle, WA, USA.
Matthew WiensUniversity of Alberta, Department of Chemistry, Edmonton, AB, Canada.
Takuya TeraiDepartment of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Japan.ORCID http://orcid.org/0000-0002-3425-3589
Kei Takahashi-YamashiroDepartment of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Japan.ORCID http://orcid.org/0000-0002-7925-6636
Roger J ThompsonCumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Timothy A BrownJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Yusuke NasuInstitute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.ORCID http://orcid.org/0000-0002-3193-9104
Minh Dang NguyenCumming School of Medicine, University of Calgary, Calgary, AB, Canada.ORCID http://orcid.org/0000-0003-4547-8617
Grant R J GordonCumming School of Medicine, University of Calgary, Calgary, AB, Canada.ORCID http://orcid.org/0000-0002-3154-7442
Sarah McFarlaneCumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Kaspar PodgorskiAllen Institute, Neural Dynamics, Seattle, WA, USA.ORCID http://orcid.org/0000-0002-0374-2005
Anthony HoltmaatDepartment of Basic Neurosciences, and Neurocenter, Faculty of Medicine, University of Geneva, Geneva, Switzerland.ORCID http://orcid.org/0000-0002-7577-0769
Robert E CampbellUniversity of Alberta, Department of Chemistry, Edmonton, AB, Canada.ORCID http://orcid.org/0000-0003-0604-092X
Alexander W LohmanCumming School of Medicine, University of Calgary, Calgary, AB, Canada. alex.lohman@ucalgary.ca.ORCID http://orcid.org/0000-0003-4363-6140

Funding

Optimization of genetically encoded voltage and neurotransmitter indicators for multiwavelength in vivo analysis of brain circuitsUM1MH136462 · NIMH · MAX PLANCK FLORIDA CORPORATION · PI Michael Z. Lin, Kaspar Podgorski · 2024 to 2026
$6.3M
Canadian HIV Trials Network, Canadian Institutes of Health Research (Réseau canadien pour les essais VIH des IRSC) PJT-195678Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada) DGECR/00271-2019NIMH NIH HHS UM1 MH136462
6 · The paper itself

Abstract

Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance GECIs are predominantly green or red fluorescent, limiting multiplexing options and efficient excitation with fixed-wavelength femtosecond lasers operating at 1030 nm. In an effort to overcome these limitations, we developed OCaMP, an orange fluorescent GECI engineered from O-GECO1 through targeted substitutions to improve calcium affinity while retaining the favorable photophysical properties of mOrange2. OCaMP exhibits improved two-photon cross-section, responsiveness, photostability, and calcium affinity relative to O-GECO1. In cultured neurons, zebrafish, and mouse cortex, OCaMP outperforms the red GECIs jRCaMP1a and jRGECO1a in sensitivity, photostability, and signal-to-noise ratio. Here we show that OCaMP, an orange fluorescent GECI, is a robust tool for high-fidelity neural imaging optimized for wavelengths above 1000 nm and a practical option within the spectral gap between existing green and red GECIs.

Indexed as

CalciumFluorescent DyesNeuronsAnimalsFluorescent Chemosensor CompoundsHumansLuminescent ProteinsMiceZebrafishCalciumFluorescent Chemosensor CompoundsFluorescent DyesLuminescent Proteins

Identifiers

PMID42331843
PMCPMC13438757

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.