ArticlePloS one2026
A minimally invasive lab protocol for fibroblasts isolation from 1-mm skin punch biopsies in pediatric patients.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Skin biopsies provide a valuable source of culturable cells for a broad range of clinical and biomedical research applications. However, conventional methods for establishing primary cell cultures typically require relatively large tissue specimens obtained through invasive procedures that involve intradermal anesthesia, suturing, and subsequent wound care. These factors are of particular concern when working with pediatric populations. To address these limitations, we developed a simplified protocol that reduces patient discomfort by minimizing the punch biopsy diameter to as little as 1 mm. Despite the markedly smaller sample size, this method consistently enables the successful establishment of primary fibroblast cultures, with no complications reported to date. This streamlined approach is compatible with basic cell culture facilities and provides a reliable source of fibroblasts for downstream applications. Overall, this protocol represents a meaningful advancement in both pediatric research and clinical practice.
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Registered trials
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