Protocol for mitochondrial DNA extraction, purity evaluation, and global methylation assessment via LC-MS/MS.

Argyri Chroni, Theodora Alexiou, John Zafeiropoulos, Vasiliki Chondrou, Argiris Symeonidis, Argyro Sgourou

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Argyri ChroniBiology Laboratory, School of Science and Technology, Hellenic Open University, Patras 26335, Greece; School of Health Sciences, Faculty of Medicine, Department of Internal Medicine, University of Patras, and "Olympion" General Clinic, Department of Hematology, Patras, Greece. Electronic address: argychroni@gmail.com.

Theodora AlexiouBiology Laboratory, School of Science and Technology, Hellenic Open University, Patras 26335, Greece. Electronic address: theodora.alexiou5@gmail.com.

John ZafeiropoulosChemistry Laboratory, School of Science and Technology, Hellenic Open University, Patras 26335, Greece. Electronic address: jzafeirop@eap.gr.

Vasiliki ChondrouBiology Laboratory, School of Science and Technology, Hellenic Open University, Patras 26335, Greece.

Argiris SymeonidisSchool of Health Sciences, Faculty of Medicine, Department of Internal Medicine, University of Patras, and "Olympion" General Clinic, Department of Hematology, Patras, Greece.

Argyro SgourouBiology Laboratory, School of Science and Technology, Hellenic Open University, Patras 26335, Greece. Electronic address: sgourou@eap.gr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Established epigenetic mechanisms, including nuclear (n)DNA methylation, are also known to regulate the mitochondrial (mt) genome despite its lack of histones. However, studies referring to evaluations of mtDNA methylation are often influenced by technical and biological confounders. We present an optimized protocol for extracting mtDNA from peripheral blood and bone marrow mononuclear cells, followed by steps for qualitative assessment to exclude nDNA contaminations and a downstream quantitative analysis of the mtDNA global methylation profile using liquid chromatography combined with mass spectrometry.

Indexed as

CancerCell BiologyCell separation/fractionationGeneticsGenomicsMass SpectrometryMetabolomicsMolecular Biology

Identifiers

PMID42329770

DOI10.1016/j.xpro.2026.104643

PMCPMC13315849

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LicenceCC BY-NC

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.