Evidence map›Paper›PMID 42329331›Full record

ArticleTropical animal health and production2026

Oral administration with silkworm pupae and fingerroot extracts affects semen quality, fertility rate, testicular function, and testosterone level in Thai native roosters.

Ruthaiporn Ratchamak, Songsak Chumpawadee, Doungnapa Promket, Thassawan Somchan, Khanitta Pengmeesri

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Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Ruthaiporn RatchamakMajor of Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Songsak ChumpawadeeMajor of Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Doungnapa PromketMajor of Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Thassawan SomchanMajor of Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Khanitta PengmeesriMajor of Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham, 44150, Thailand. khanitta.c@msu.ac.th.ORCID http://orcid.org/0009-0005-7312-3452

Funding

Thailand Science Research and Innovation FF-670636
6 · The paper itself

Abstract

The populations of native roosters exhibit a reduced capacity for reproduction because of thermal stress, which adversely influences the quality of semen and the production of sperm. Nutritional strategies using natural bioactive compounds may boost male reproductive function, with silkworm pupae as a high-quality protein source and fingerroot containing antioxidant phytochemicals This study evaluated the effects of oral silkworm pupae and fingerroot extract on semen quality, fertility, testicular histology, and serum testosterone. Roosters were randomly assigned into three groups: control, silkworm pupae-supplemented (500 mg/day), and fingerroot-supplemented (500 mg/day). Proximate analysis revealed silkworm pupae is rich in protein (53.7%) and lipids (32.1%), while fingerroot exhibited high antioxidant activity through phenolics and flavonoids. Results demonstrated significant improvements (P < 0.05) in progressive sperm motility (68.54 ± 1.33%) and testosterone levels (6.86 ± 1.38 ng/mL) in the silkworm pupae group compared to controls. Fingerroot supplementation significantly increased sperm concentration (3.89 × 10⁹ sperm/mL; P < 0.05). Furthermore, silkworm pupae improved seminiferous tubule width, germinal epithelial growth, and spermatogenic activity, with significantly higher testosterone levels compared to the control group (2.21 ± 0.51 ng/mL; P < 0.05). In conclusion, silkworm pupae and fingerroot extract effectively improved semen quality and testosterone levels in Thai native roosters. These findings highlight their potential as promising natural dietary supplements to enhance reproductive performance in sustainable indigenous poultry farming.

Indexed as

BombyxChickensFertilityTestisTestosteroneAdministration, OralAnimal FeedAnimalsDietMalePlant RootsPupaSemen AnalysisThailandTestosteroneFertilityFingerroot extractReproductive performanceSilkworm pupae

Identifiers

PMID42329331

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.