Evidence map›Paper›PMID 42327153›Full record

ArticlebioRxiv : the preprint server for biology2026

Revealing the spatiotemporal dynamics of methionine metabolism with a genetically encoded single-fluorophore biosensor.

Katarina A Cohen, Arnav Jhawar, Gilberto Garcia, Naiara Goni, Megan Chen, Athena Alcala, Ryo Higuchi-Sanabria, Danielle L Schmitt

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Katarina A CohenDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, 90095 USA.ORCID 0000-0001-7769-7414
Arnav JhawarDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, 90095 USA.
Gilberto GarciaLeonard Davis School of Gerontology, University of Southern California, Los Angeles, CA 90089 USA.ORCID 0000-0001-9959-650X
Naiara GoniDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, 90095 USA.
Megan ChenDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, 90095 USA.
Athena AlcalaLeonard Davis School of Gerontology, University of Southern California, Los Angeles, CA 90089 USA.ORCID 0009-0001-6537-7250
Ryo Higuchi-SanabriaLeonard Davis School of Gerontology, University of Southern California, Los Angeles, CA 90089 USA.ORCID 0000-0001-8936-5812
Danielle L SchmittDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, 90095 USA.ORCID 0000-0002-7343-2125

Funding

Imaging Spatiotemporal Regulation of Acetyl-CoADP2GM154012 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Danielle Lynanne Schmitt · 2023 to 2026
$2.2M
When actin’s not actin like actin: Nuclear actin impacts transcription and agingR01AG079806 · NIA · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Ryo Higuchi-Sanabria · 2024 to 2026
$1.9M
NIA NIH HHS R01 AG079806NIGMS NIH HHS DP2 GM154012
6 · The paper itself

Abstract

Methionine is an essential amino acid, used for protein synthesis, redox homeostasis, and methylation reactions throughout the cell. However, the compartmentalized dynamics of methionine have remained elusive, due to a lack of available tools to measure methionine with high spatial and temporal resolution. To address this limitation, we have developed a single fluorescent protein-based methionine optical reporter (Meteor) which reports subcellular changes in methionine with high dynamic range. Using Meteor, we demonstrate the subcellular uptake of methionine in multiple cell lines into several locations, including the mitochondrial matrix. Furthermore, we use Meteor to illuminate the dynamics of the methionine cycle in the cytoplasm and nucleus, finding cancer cells can rapidly increase methionine from metabolic precursors in both locations. Finally, demonstrated that Meteor can be used to visualize methionine dynamics

Identifiers

PMID42327153
PMCPMC13277817

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.