ArticleACS measurement science au2026
From Gold to Polymer: Latex Bead-Assisted CRISPR-Cas12a Platform for Next-Generation Protein Diagnostics.
Article in ACS measurement science au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sensitive and accurate detection of protein biomarkers in clinical samples is crucial for early disease diagnosis and monitoring treatment outcomes. Although gold nanoparticle (AuNP)-assisted CRISPR-Cas12a biosensors have shown promise, their practical applications have been restricted by limitations of AuNPs, such as costly and complex synthesis, tendency to aggregate, and nonspecific adsorption of various unwanted species. To address these limitations, herein, we report a CRISPR-Cas12a-based fluorescence biosensor for the detection of a well-established and clinically significant inflammatory biomarker, interleukin-6 (IL-6), which employs latex beads instead of AuNPs as a transducer and signal amplifier. By the combined use of IL-6 detection antibody-functionalized magnetic beads, dual-functionalized latex beads, which carry both IL-6 detection antibodies and multiple dsDNA strands per antibody molecule, and the Cas12a-crRNA system, our method was able to detect IL-6 with a limit of detection (LOD) reaching as low as 0.76 pg/mL. Moreover, our sensor was highly selective: nontarget proteins such as human serum albumin (HSA), bovine serum albumin (BSA), C-reactive protein (CRP), procalcitonin (PCT), and IL-2β would not interfere with the detection of IL-6. In addition, the practical application of this sensor was assessed by successfully analyzing simulated serum samples. This study shows that latex beads can serve as an alternative to gold nanoparticles for CRISPR-based protein diagnostics. This approach improves stability and specificity while maintaining a high level of sensitivity for early disease diagnosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.