Linking Nanoscale Film Properties to Electrochemical Response in Cytochrome P450 Membrane Enzyme Films.

Silan Bhandari, Matthew Dixon, Charuksha Walgama, Rajasekhara Nerimetla, Sadagopan Krishnan

Abstract read

In one paragraph

Article in ACS measurement science au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Silan BhandariDepartment of Chemistry, Oklahoma State University, Stillwater, Oklahoma 74078, United States.

Matthew DixonNanoscience Instruments, 10008 S. 51st Street, Suite 110, Phoenix, Arizona 85044, United States.

Charuksha WalgamaDepartment of Physical & Applied Sciences, University of Houston-Clear Lake, Houston, Texas 77058, United States.ORCID https://orcid.org/0000-0002-5549-0436

Rajasekhara NerimetlaDepartment of Chemistry, Oklahoma State University, Stillwater, Oklahoma 74078, United States.

Sadagopan KrishnanDepartment of Chemistry, Oklahoma State University, Stillwater, Oklahoma 74078, United States.ORCID https://orcid.org/0000-0002-9968-2712

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membrane-bound human liver cytochrome P450 (CYP) enzymes catalyze key steps in xenobiotic metabolism, yet their integration into analytical devices depends critically on surface chemistry and film mechanics. Bacterial membrane preparations ("bactosomes") provide abundant CYP sources for in vitro measurements and biosensors. Here, cystamine self-assembled monolayers (SAMs) on gold-coated quartz were compared with polyethylenimine (PEI)-coated gold for immobilizing CYP bactosomes. Quartz crystal microbalance with dissipation monitoring (QCM-D) tracked adsorption and film evolution, enabling quantitative estimates of the mass loading, thickness, and viscoelastic properties. After washing, bactosomes/SAM reached ∼113 nm thickness and 11.9 μg cm

Indexed as

adsorptionbiomembraneselectrochemical responsenanoscale filmsquartz crystal microbalancesurface modification

Identifiers

PMID42326857

DOI10.1021/acsmeasuresciau.5c00129

PMCPMC13281187

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.