ArticleInfectious diseases & immunity2025
Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared.
Article in Infectious diseases & immunity, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
2 authors.
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Abstract
Due to its technological advantages, single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals. The preparation of a high-quality single-cell suspension from solid tissues is a critical step prior to single-cell RNA sequencing. Based on a brief overview of the steps involved in preparing cell suspensions from solid tissues for single-cell RNA sequencing, we present a detailed analysis of the pivotal steps in the preparation process, including enzymatic digestion, mechanical dissociation, and cell viability evaluation. The clarification of these experimental details will greatly help us obtain high-quality single-cell sequencing data from solid tissues.
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Registered trials
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