Evidence map›Paper›PMID 42325577›Full record

ArticleiScience2026

A key role for the exoribonuclease XRN1 in regulating the hepatitis B viral transcriptome.

Senko Tsukuda, Nadina Wand, James M Harris, Olivia Dobrica, Medhi Boutasbih, Xiaxuan Zhu, Peter A C Wing, Peter Balfe, Jane A McKeating

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Senko TsukudaChinese Academy of Medical Sciences Oxford Institute, University of Oxford, Oxford, UK.
Nadina WandNuffield Department of Medicine, University of Oxford, Oxford, UK.
James M HarrisNuffield Department of Medicine, University of Oxford, Oxford, UK.
Olivia DobricaNuffield Department of Medicine, University of Oxford, Oxford, UK.
Medhi BoutasbihNuffield Department of Medicine, University of Oxford, Oxford, UK.
Xiaxuan ZhuNuffield Department of Medicine, University of Oxford, Oxford, UK.
Peter A C WingChinese Academy of Medical Sciences Oxford Institute, University of Oxford, Oxford, UK.
Peter BalfeNuffield Department of Medicine, University of Oxford, Oxford, UK.
Jane A McKeatingChinese Academy of Medical Sciences Oxford Institute, University of Oxford, Oxford, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chronic hepatitis B virus (HBV) is a leading cause of liver disease and hepatocellular carcinoma that persists as a DNA mini-chromosome and replicates via genomic RNA intermediates. While HBV replication has been extensively studied, mechanisms governing viral RNA degradation remain unclear. Here, we identify a key role for the 5'-3' exoribonuclease XRN1 in regulating the HBV transcriptome. Pharmacologic inhibition or genetic ablation of XRN1 increased HBV subgenomic RNAs without affecting pregenomic RNA (pgRNA) levels. Long-read RNA-seq showed that subgenomic RNAs, but not pgRNA, are degraded by XRN1, consistent with differential 5' cap modification and processing (P-) body localization. HBV infection increased P-body number and size, and delivery of synthetic pgRNA demonstrated that active replication, not RNA expression alone, alters the abundance of P-bodies. These findings identify XRN1 as a key regulator of HBV RNA turnover and uncover a mechanism for viral replication to remodel host RNA dynamics.

Indexed as

Molecular biologyVirology

Identifiers

PMID42325577
PMCPMC13276156

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.