ArticleiScience2026
Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples.
Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
15 authors.
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Abstract
Emerging imaging strategies, such as tissue clearing and advanced light-sheet modalities, are transforming the visualization of cell differentiation, tissue reorganization, and morphogenesis in both model and non-model organisms, thereby driving future discoveries of conserved developmental programs and species-specific innovations. In this study, we present a versatile Bessel light sheet microscopy (BLX) system compatible with both immersion and capped air objectives, optimized for whole-mount three-dimensional imaging of cleared tissues. We demonstrate that autofluorescence-based light-sheet imaging enables reliable visualization of CUBIC-R-cleared mouse embryos, revealing microanatomical features such as nephrons during developmental stages. To support solvent-based clearing protocols, we designed a protective lens cap that shields objectives from solvent erosion and economically converts standard air objectives into high-performance immersion objectives. Using this capped BLX configuration, we achieved cellular-resolution imaging of whole PEGASOS-cleared mouse brains. The system's simplified design and open-source availability promote broader adoption for diverse applications in developmental biology.
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