Lysozyme revisited: evaluating models of a reference protein in structural biology.

Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Mariusz Jaskolski, Wladek Minor

Abstract read

In one paragraph

Article in Current research in structural biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Comparative assessment of crystallographic and cryo-EM models in the Protein Data Bank.FEBS open bio · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Alexander WlodawerLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, 20892, USA.

Pawel RubachDepartment of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, 22908, USA.

Zbigniew DauterHKL Research, Charlottesville, VA, 22908, USA.

Wojciech DecDepartment of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, 22908, USA.

Dariusz BrzezinskiInstitute of Computing Science, Poznan University of Technology, Poznan, Poland.

Mariusz JaskolskiInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.

Wladek MinorDepartment of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, 22908, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have evaluated the quality of over 1200 crystal structures of hen egg-white lysozyme (HEWL) deposited in the Protein Data Bank (PDB). These structures, collected over nearly 50 years, vary in quality, despite all representing essentially the same small enzyme consisting of 129 amino acid residues. Some of the entries originated from studies of the binding of small-molecule ligands to HEWL, whereas the majority of deposits represent the outcomes of tests of new experimental approaches to crystallization and data collection and/or evaluations of new computational protocols. We found no correlation between

Indexed as

Artificial intelligence (AI)Benchmark proteinsData integrityReference proteinStructure qualityTraining set optimization

Identifiers

PMID42325258

DOI10.1016/j.crstbi.2026.100192

PMCPMC13279888

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LicenceCC BY-NC

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.