Evidence map›Paper›PMID 42324700›Full record

ArticleMicrobiologyOpen2026

Molecular Characterization and Antifungal Profiling of Nine Phenotypic Aspergillus nidulans Isolates: A Case Series From North India.

Aishwarya Nikhil, Atul Kumar Tiwari, Pearl Parashar, Mohit Bhatia, Ragini Tilak, Deepak Kumar, Sudhir Kumar Singh, Roger J Narayan, Munesh K Gupta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MicrobiologyOpen, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Aishwarya NikhilMycology Research Group, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.ORCID 0009-0000-0399-8505
Atul Kumar TiwariSchool of Chemistry, Tel Aviv University, Tel Aviv, Yafo, Israel.
Pearl ParasharMycology Research Group, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Mohit BhatiaDepartment of TB and Respiratory Medicine, Sir Sunderlal Hospital (BHU), Varanasi, Uttar Pradesh, India.
Ragini TilakMycology Research Group, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Deepak KumarViral Research Diagnostic Laboratory, Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Sudhir Kumar SinghViral Research Diagnostic Laboratory, Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Roger J NarayanJoint Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina, USA.
Munesh K GuptaMycology Research Group, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.

Funding

Banaras Hindu University III/2022-23/49235
6 · The paper itself

Abstract

Aspergillus nidulans is a fungal pathogen that causes respiratory issues in individuals with compromised immune systems. It is identified through culture characteristics and microscopic features such as Cleistothecia and Hülle Cells. However, similar traits are found in cryptic Aspergillus species, such as stellatus, cristatus, and oryzae. In a case series from a hospital in North India, A. nidulans strains isolated from patients with pulmonary aspergillosis were phenotypically identified and then underwent molecular characterization through sequencing of the amplified ITS1-5.8S rDNA-ITS2 region and antifungal susceptibility testing (AST) according to the CLSI M38A3 guidelines. The molecular characteristics and antifungal profiles of the nine phenotypic A. nidulans were as follows: A. stellatus (n = 5), A. nidulans (n = 2), A. cristatus (n = 1), and A. oryzae (n = 1). Phylogenetic analysis revealed a close relationship between A. stellatus, A. nidulans, and A. cristatus, while A. oryzae showed significant divergence. Furthermore, the minimum inhibitory concentration (MIC) of antifungals was the lowest for caspofungin, followed by voriconazole. However, a higher amphotericin B MIC (2 µg/mL) was observed for A. stellatus. Thus, sequencing the ITS1-5.8S rDNA-ITS2 region can accurately identify cryptic species with superior taxonomic resolution. Additionally, the MIC of amphotericin B against A. stellatus underscores the importance of precise molecular identification and antifungal MIC profiling in each case of pulmonary aspergillosis.

Indexed as

Antifungal AgentsAspergillus nidulansPulmonary AspergillosisCluster AnalysisDNA, FungalDNA, RibosomalDNA, Ribosomal SpacerHumansIndiaMicrobial Sensitivity TestsMolecular Sequence DataPhylogenyRNA, Ribosomal, 5.8SSequence Analysis, DNAAntifungal AgentsDNA, FungalDNA, RibosomalDNA, Ribosomal SpacerRNA, Ribosomal, 5.8SAspergillus nidulansAspergillus stellatusChronic pulmonary aspergillosiscryptic speciesITS1‐5.8SrDNA

Identifiers

PMID42324700
PMCPMC13284283

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.