ArticleMicrobiologyOpen2026
The Role of Neonatal Ward Environment as a Reservoir for the Dissemination of Multidrug-Resistant Klebsiella pneumoniae in Algeria.
Article in MicrobiologyOpen, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors.
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Abstract
Klebsiella pneumoniae is a major cause of hospital-acquired infections and contributes to high mortality. We examined the molecular epidemiology, antibiotic resistance, and virulence features of K. pneumoniae from the neonatal ward environment of a hospital in Chlef, Algeria, using whole-genome sequencing (WGS). Antibiotic susceptibility was assessed with the Vitek 2 automated system (AST-N365 card). Genomic DNA was extracted with the GF-1 kit, and WGS was performed by GENEWIZ Europe using the NEBNext Ultra II DNA Library Prep Kit. Species identification and virulence genes were determined using Kleborate, antimicrobial resistance genes were detected with AMRfinder Plus. From 9790 publicly available K. pneumoniae genomes, the 112 closest matches were selected, and core-genome MLST and a minimum-spanning tree were generated in SeqSphere + v10.5.04 using a MLST and cgMLST scheme. All isolates were multidrug-resistant, and WGS identified 22 resistance genes. Core genes detected across all samples included the bla
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