Evidence map›Paper›PMID 42323582›Full record

ArticleBMC plant biology2026

Clone and characterization of a cytochrome P450 gene for drought tolerance in rice.

Wenqiang Liu, Zuwu Chen, Zheng Dong, Liang Guo, Biaoren Yang, Licheng Liu, Sanxiong Liu, Xiaowu Pan

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Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Wenqiang LiuState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Zuwu ChenState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Zheng DongState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Liang GuoState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Biaoren YangState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Licheng LiuState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Sanxiong LiuState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Xiaowu PanState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, 410125, China. panxiaowu@hunaas.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDrought is a major abiotic stress limiting rice production worldwide. Identifying genes that enhance drought tolerance is essential for breeding resilient varieties.

resultsIn this study, we report the map-based cloning and functional characterization of DT1, a novel cytochrome P450 gene conferring drought tolerance in rice. Using near isogenic lines (NILs) derived from a cross between Xiang743 and Katy, we delimited DT1 into a 115 kb interval on chromosome 3, where contains 18 open read frames (ORFs). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis identified Os03g55250 as the candidate gene. Clustered regularly interspaced short palindromic repeats-associated nuclease 9 (CRISPR/Cas9) knockout mutants of Os03g55250 exhibited increased drought sensitivity, while overexpression lines showed enhanced drought tolerance, confirming that Os03g55250 was the target gene and positively regulates drought resistance. DT1 was mainly expressed in stems, leaves, and leaf sheaths, and the DT1 protein localized in the endoplasmic reticulum. Haplotype analysis identified Hap1 as a favorable allele in japonica rice.

conclusionsOur findings provide a promising genetic resource for breeding drought-resistant rice varieties and offers new insights into the role of P450 genes in abiotic stress adaptation.

Indexed as

Cytochrome P-450 Enzyme SystemOryzaPlant ProteinsCloning, MolecularDrought ResistanceDroughtsGene Expression Regulation, PlantGenes, PlantPlants, Genetically ModifiedStress, PhysiologicalCytochrome P-450 Enzyme SystemPlant ProteinsCloneDrought toleranceP450 geneRice (Oryza sativa L.)

Identifiers

PMID42323582
PMCPMC13536573

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