Protocol for canalostomy in rats to enable inner ear injection.

Lei Zhang, Hanwen Zhou, Wenjie Liu, Siyue Wang, Huiyue Wang, Jinyu Wang, Ning Cong, Jing Wang

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Lei ZhangDepartment of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, Shanghai 200031, China. Electronic address: lei.zhang.research@gmail.com.

Hanwen ZhouDepartment of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.

Wenjie LiuDepartment of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.

Siyue WangDepartment of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.

Huiyue WangDepartment of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.

Jinyu WangDepartment of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.

Ning CongDepartment of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.

Jing WangDepartment of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, Shanghai 200031, China. Electronic address: wangjing6437@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for precise inner ear access in rats using a refined canalostomy technique. We describe steps for surgical exposure of the mastoid bone, localization of the target semicircular canal, fenestration using a cranial drill, and quantitative microinjection of agents. We further validate this approach by presenting fluorescence-based assessment results. This standardized approach enables reproducible delivery of chemicals or viruses into the inner ear.

Indexed as

GeneticsModel OrganismsNeuroscience

Identifiers

PMID42322612

DOI10.1016/j.xpro.2026.104645

PMCPMC13314900

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.