ArticleInternational journal of clinical oncology2026
PVA sponge method improves detection efficiency of cfDNA in plasma.
Article in International journal of clinical oncology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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15 authors.
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Abstract
backgroundCell-free DNA (cfDNA) analysis is an important tool in molecular diagnostics and translational research. However, conventional cfDNA extraction from plasma is often labor-intensive, and inefficient due to interference from blood-derived substances. We developed a novel cfDNA sample preparation method using polyvinyl alcohol (PVA) sponges that enables direct qPCR without extraction or purification.
methodsPVA sponge pieces were prepared and used to dry plasma or DNA standard solutions. After drying, the sponge was directly added to the qPCR mixture. Recovery yields were evaluated using human genomic DNA and 200 bp synthetic DNA at various concentrations and compared with two commercial extraction kits: QIAamp DNA Blood Mini (DBM) and QIAamp MinElute ccfDNA (MEC). Clinical plasma samples from 10 patients were also analyzed.
resultsPVA sponges did not interfere with qPCR amplification, achieving recovery yields of 90.7% - 105.3% for genomic DNA. In plasma-spiked samples, qPCR inhibition was mitigated by using PVA sponges, resulting in improved amplification curves and reduced Ct variability. For low-concentration synthetic DNA (10
conclusionThe PVA sponge method enables efficient and rapid cfDNA quantification directly from plasma without extraction. Its simplicity, speed, and cost-effectiveness make it a promising tool for liquid biopsy applications in clinical practice.
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