Evidence map›Paper›PMID 42322472›Full record

ArticleActa parasitologica2026

Molecular Identification of Cytochrome b Mutations in Buparvaquone-Unresponsive Theileria annulata Isolates from Khorasan-Razavi, Iran.

Emad Changizi, Amin Esmaeilian, Hesam Ashareyoun, Gholamreza Razmi, Hamid Staji, Iraj Ashrafi Tamai

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Article in Acta parasitologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Emad ChangiziPathobiology Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran. echangizi@gmail.com.
Amin EsmaeilianGraduated From the Master's Degree in Veterinary Parasitology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Hesam AshareyounDVSc in Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Gholamreza RazmiPathobiology Department, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Hamid StajiPathobiology Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Iraj Ashrafi TamaiPathobiology Department, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

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6 · The paper itself

Abstract

introductionThe failure of conventional therapies for bovine theileriosis in Khorasan-Razavi province and other regions of Iran has led to significant economic losses and raised concerns about rural livestock sustainability and national food security. This study identified specific cytochrome b mutations in Theileria annulata isolates collected from cattle showing clinical unresponsiveness to buparvaquone treatment in the Khorasan-Razavi province of Iran. MATERIALS AND

methodsBlood samples were collected, and parasite DNA was extracted and amplified via PCR using specific primers.

resultsSequencing and subsequent BLAST analysis revealed two novel cytochrome b variants, OQ230576 and OQ230577, registered in the NCBI GenBank. These variants exhibited multiple non-synonymous mutations compared to sensitive reference strains from Iran (MH248139/1) and Ankara. The OQ230576 sequence demonstrated six to ten mutations, while OQ230577 exhibited eight to ten mutations, suggesting structural alterations linked to drug resistance.

conclusionsThese findings confirm the emergence and spread of resistant Theileria annulata strains in the studied regions. The progressive decline in treatment efficacy underscores the urgent need for alternative therapeutic approaches and more robust disease management strategies to safeguard livestock productivity and rural economies.

Indexed as

Antiprotozoal AgentsCytochromes bDrug ResistanceMutationNaphthoquinonesTheileria annulataTheileriasisAnimalsCattleIranPolymerase Chain ReactionSequence Analysis, DNAAntiprotozoal AgentsbuparvaquoneCytochromes bNaphthoquinonesBovine TheileriosisDrug resistanceFood securityMutations

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PMID42322472

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