Evidence map›Paper›PMID 42322463›Full record

ArticleVeterinary research communications2026

The applicability of pregnancy diagnosis using interferon-stimulated genes in embryo-transferred Japanese Black cattle.

Hitomi Yoshino, Toh-Ichi Hirata, Hideo Matsuda, Tadayuki Yamanouchi, Yutaka Hashiyada, Kei Imai, Toru Takahashi, Toshina Ishiguro-Oonuma, Kazuyoshi Hashizume, Keiichiro Kizaki

Abstract read
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Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hitomi YoshinoGraduate School of Veterinary Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020- 8550, Japan.
Toh-Ichi HirataAnimal Feed Science Center, Faculty of Agriculture, Iwate University, Shizukuishi, Iwate, 020-0581, Japan.
Hideo MatsudaNational Livestock Breeding Center, Nishigo, Fukushima, 961-8061, Japan.
Tadayuki YamanouchiNational Livestock Breeding Center, Nishigo, Fukushima, 961-8061, Japan.
Yutaka HashiyadaDepartment of Bioproduction Science, Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Kei ImaiDepartment of Sustainable Agriculture, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.
Toru TakahashiGraduate School of Veterinary Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020- 8550, Japan.
Toshina Ishiguro-OonumaGraduate School of Veterinary Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020- 8550, Japan.
Kazuyoshi HashizumeGraduate School of Veterinary Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020- 8550, Japan.
Keiichiro KizakiGraduate School of Veterinary Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020- 8550, Japan. kizaki@iwate-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The early pregnancy diagnosis method by measuring interferon-stimulated genes (ISGs) in peripheral blood can be used in cattle after artificial insemination (AI) with high accuracy. ISGs are genes whose expression is induced by interferon tau (IFNT) in the trophectoderm of the conceptus during early pregnancy in cattle. We evaluated the applicability of ISG-based pregnancy diagnosis in embryo-transferred (ET) cattle. ISG levels in the peripheral blood were measured on days 19 and 21 of gestation in ET cattle, and diagnostic accuracy was calculated. The diagnostic potential of ISG was assessed using previously established threshold values for AI cattle. The diagnostic threshold values for ET cattle were also calculated using the Youden index from the receiver operating characteristic curve. Regardless of the threshold values used, the results showed significantly higher ISG expression in pregnant than in non-pregnant cattle on day 21, but not on day 19. Pregnancy diagnosis in ET cattle resulted in reduced accuracy, particularly on day 19, compared to AI cattle. These findings suggest that the causes of non-conception may differ between AI and ET cattle. These findings highlight the limitations of using ISG-based early pregnancy diagnosis during the preimplantation period in ET cattle.

Indexed as

Embryo TransferInterferonsInterferon Type IPregnancy ProteinsPregnancy TestsAnimalsCattleFemaleInsemination, ArtificialPregnancyInterferonsinterferon tauInterferon Type IPregnancy ProteinsEmbryo transferInterferon-stimulated genesPregnancy diagnosis

Identifiers

PMID42322463
PMCPMC13283135

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.