Evidence map›Paper›PMID 42321830›Full record

ArticleMicrobial cell factories2026

Exploring the metabolic burden of surfactin biosynthesis and the metabolic costs of srfA operon expression in Bacillus subtilis.

Elvio Henrique Benatto Perino, Eric Hiller, Maliheh Vahidinasab, Sandra Moneta, Bahar Abrishamchi, Kowsala Nagendran, Philipp Hubel, Jens Pfannstiel, Rudolf Hausmann

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In one paragraph

Article in Microbial cell factories, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Elvio Henrique Benatto Perino *Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstrasse 12, 70599, Stuttgart, Germany. eperino@uni-hohenheim.de.ORCID https://orcid.org/0000-0003-0372-260X
Eric Hiller *Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstrasse 12, 70599, Stuttgart, Germany. eric.hiller@uni-hohenheim.de.
Maliheh VahidinasabDepartment of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstrasse 12, 70599, Stuttgart, Germany.
Sandra MonetaDepartment of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstrasse 12, 70599, Stuttgart, Germany.
Bahar AbrishamchiDepartment of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstrasse 12, 70599, Stuttgart, Germany.
Kowsala NagendranDepartment of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstrasse 12, 70599, Stuttgart, Germany.
Philipp HubelCore Facility Hohenheim, Mass Spectrometry Unit, University of Hohenheim, August-von-Hartmann-Str. 3, 70599, Stuttgart, Germany.
Jens PfannstielCore Facility Hohenheim, Mass Spectrometry Unit, University of Hohenheim, August-von-Hartmann-Str. 3, 70599, Stuttgart, Germany.
Rudolf HausmannDepartment of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstrasse 12, 70599, Stuttgart, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The biosynthesis of surfactin, a potent lipopeptide biosurfactant produced by Bacillus subtilis, imposes a significant metabolic burden on the host organism. Understanding the metabolic costs associated with surfactin production, specifically the ATP demand and precursor diversion resulting from the expression of the large srfAA-AD operon (srfA operon), is crucial for optimizing production strains. In this study, the metabolic burden and growth impacts associated with surfactin biosynthesis in B. subtilis were quantitatively evaluated by comparing a reference surfactin-producing strain (BMV9) with two mutant strains: BMV12, which lacks the srfA operon, and BMV33, which retains the srfA operon but lacks the sfp gene. Our analysis included theoretical calculations of ATP and NADPH + H

Indexed as

Bacillus subtilisBacterial ProteinsLipopeptidesOperonPeptides, CyclicBiosurfactantsCarbonGene Expression Regulation, BacterialPeptide SynthasesBacterial ProteinsCarbonLipopeptidesnon-ribosomal peptide synthasePeptides, CyclicPeptide Synthasessurfactin peptideBacillus subtilisKinetic modellingLipopeptidesMetabolic burdenNRPSSurfactin

Identifiers

PMID42321830
PMCPMC13289377

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.