Evidence map›Paper›PMID 42321513›Full record

ArticleCell biology and toxicology2026

Mechanism of palytoxin-induced ferroptosis in HaCaT cells via targeting TrxR1.

Cheng Zhong, Zihan Zhao, Dong Chen, Longze Lü, Wanxin Ren, Xinhao Li, Bowen Deng, Chengming Yang, Yao Yang, Lianghua Wang and 1 more

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Article in Cell biology and toxicology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Cheng Zhong *Naval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China.
Zihan Zhao *Naval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China.
Dong Chen *Naval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China.
Longze LüNaval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China.
Wanxin RenNaval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China.
Xinhao LiNaval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China.
Bowen DengNaval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China.
Chengming YangNaval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China.
Yao YangNaval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China. yangyao2017@foxmail.com.
Lianghua WangNaval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China. lhwang@smmu.edu.cn.
Mingjuan SunNaval Medical University, No.800, Xiangyin Road, Shanghai, 200433, China. sunmj@smmu.edu.cn.ORCID https://orcid.org/0009-0004-9185-4024

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

PLTX (Palytoxin) is a fatty-chain polyether marine biotoxin originally produced by benthic dinoflagellates, particularly Ostreopsis ovata and Ostreopsis siamensis. Via the marine food web, PLTX bioaccumulates in numerous organisms such as corals and shellfish, and ranks among the most lethal non-proteinaceous natural compounds described to date. Thus far, its toxicological mechanism remains unclear, and no specific clinical antidote is available. In this study, we employed a limited proteolysis‑based chemical proteomics approach (Lip‑SMap) to analyze the protection of protease cleavage sites conferred by PLTX binding. Combined with DIA (data‑independent acquisition) mass spectrometry in HaCaT (human immortalized keratinocyte) cells, we identified TrxR1 (thioredoxin reductase 1) as an intracellular direct target of PLTX. Molecular docking simulations revealed that PLTX forms stable hydrogen bonds with the active‑site residues Q230 and N234 of TrxR1. BLI (Biolayer interferometry) further confirmed a moderate‑to‑high affinity interaction between them (KD = 5.415 × 10

Indexed as

AcrylamidesFerroptosisThioredoxin Reductase 1Cnidarian VenomsGlutathioneHaCaT CellsHumansMolecular Docking SimulationPolyether ToxinsReactive Oxygen SpeciesAcrylamidesCnidarian VenomsGlutathionepalytoxinPolyether ToxinsReactive Oxygen SpeciesThioredoxin Reductase 1TXNRD1 protein, humanCystine/GSH/GPx4 axisFerroptosisLip-SMapPalytoxinThioredoxin reductase 1

Identifiers

PMID42321513

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.