Age (juvenile to aged) and sex effects on in vitro CYP2A6 enzyme activity in African green monkeys using nicotine and coumarin.

Weilong Gu, Rachel F Tyndale

Abstract read

In one paragraph

Article in Drug metabolism and disposition: the biological fate of chemicals, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Weilong GuDepartment of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada.

Rachel F TyndaleDepartment of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada; Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health (CAMH), Toronto, Ontario, Canada; Department of Psychiatry, University of Toronto, Toronto, Ontario, Canada. Electronic address: r.tyndale@utoronto.ca.

Funding

Variable brain oxycodone metabolism alters drug effectR01DA043526 · NIDA · UNIVERSITY OF TORONTO · PI TYNDALE, RACHEL FYNVOLA · 2018 to 2022

$1.5M

NIDA NIH HHS R01 DA043526

6 · The paper itself

Abstract

CYP2A6 is the principal human enzyme metabolizing coumarin and nicotine; it demonstrates wide variation from genetics and environmental regulation, complicating the determination of human developmental profile and sex-related differences. African green monkeys (AGMs), with comparable CYP2A6 activity to humans, of both sexes across 5 developmental stages (N = 56) were used to investigate age and sex effects on CYP2A6 activity using 7-hydroxycoumarin formation from coumarin, and cotinine formation from nicotine. Age and sex differences were determined using two-way ANOVAs and post hoc tests. Age impacted microsomal protein per gram liver (determined by cytochrome P450 oxidoreductase activity). Age and sex differences, with interactions, were observed in liver and body weight, whereby older AGMs and males were heavier for both. Pooled microsomes per age-sex group were used to determine the kinetic parameters for 7-hydroxycoumarin (overall K

Indexed as

AgingCoumarinsCytochrome P-450 CYP2A6NicotineAge FactorsAnimalsChlorocebus aethiopsCotinineFemaleLiverMaleMicrosomes, LiverSex CharacteristicsSex FactorsUmbelliferones7-hydroxycoumarinCotininecoumarinCoumarinsCytochrome P-450 CYP2A6NicotineUmbelliferonesAfrican green monkeyCoumarinCYP2A6Drug metabolismNicotine

Identifiers

PMID42320234

DOI10.1016/j.dmd.2026.100335

PMCPMC13494167

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.