Evidence map›Paper›PMID 42319833›Full record

ArticleSTAR protocols2026

Protocol for connector-enabled multigene pathway assembly using Golden Gate Assembly and yeast recombination.

Min-Jun Seong, Jonghyeok Shin, Dae-Hee Lee

Abstract read
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Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Min-Jun SeongSynthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
Jonghyeok ShinSynthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Department of Food Science and Biotechnology, College of Life Sciences, Sejong University, Seoul 05006, Republic of Korea; Carbohydrate Bioproduct Research Center, Sejong University, Seoul 05006, Republic of Korea. Electronic address: jhshin@sejong.ac.kr.
Dae-Hee LeeSynthetic Biology Research Center and Korea Biofoundry, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea; Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea; Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon-si, Gyeonggi-do 16419, Republic of Korea. Electronic address: dhlee@kribb.re.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for multigene pathway construction in Saccharomyces cerevisiae using a connector-enabled workflow that integrates Golden Gate Assembly with yeast homologous recombination. We provide procedures for assembling Level 1 transcriptional unit (TU) fragments using standardized connectors and directly co-transforming them into S. cerevisiae to form Level 2 multigene plasmids. Compared to conventional approaches, this protocol significantly reduces procedural complexity, minimizes dependence on operator expertise, and substantially accelerates workflow timelines. For complete details on the use and execution of this protocol, please refer to Seong et al.

Indexed as

Biotechnology and bioengineeringMolecular BiologySystems biology

Identifiers

PMID42319833
PMCPMC13312114

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.