Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.

Rebekah J Nicholson, J Alan Maschek, Trevor Lonergan, Scott A Summers

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Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Rebekah J NicholsonDepartment of Nutrition and Integrative Physiology, University of Utah College of Health, Salt Lake City, UT, USA; Center for Metabolic Health, University of Utah, Salt Lake City, UT, USA.

J Alan MaschekCenter for Metabolic Health, University of Utah, Salt Lake City, UT, USA; Metabolomics and Lipidomics Core Facility, University of Utah, Salt Lake City, UT, USA. Electronic address: alan.maschek@pharm.utah.edu.

Trevor LonerganMetabolomics and Lipidomics Core Facility, University of Utah, Salt Lake City, UT, USA.

Scott A SummersDepartment of Nutrition and Integrative Physiology, University of Utah College of Health, Salt Lake City, UT, USA; Center for Metabolic Health, University of Utah, Salt Lake City, UT, USA. Electronic address: scott.a.summers@health.utah.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Urinary sphingolipids may serve as biomarkers of renal diseases. Here, we present a high-throughput, automated protocol for solid-phase extraction and tandem mass spectrometry (LC-MS/MS) quantification of lipids from urine. We describe steps for sample preparation and lipid extraction, and then detail procedures for targeted LC-MS/MS analysis of urinary sphingolipids. In this protocol, we focus on the analysis of urine samples; however, this platform can be optimized for alternative biospecimens. Additionally, this method allows for tandem preparation of metabolomic and lipidomic samples. For complete details on the use and execution of this protocol, please refer to Nicholson et al.

Indexed as

Health SciencesHigh Throughput ScreeningMetabolismMetabolomicsProtocols in Metabolomics and Lipidomics

Identifiers

PMID42319831

DOI10.1016/j.xpro.2026.104644

PMCPMC13312081

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.