Evidence map›Paper›PMID 42315821›Full record

ArticleDaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences2026

Bio-guided identification of novel antiparasitic natural products from Gardenia imperialis K. Schum (Rubiaceae).

Christelle Wayoue Kom, Sylvester Osei Bobbie, Darline Dize, Cyrille Armel Njanpa Ngansop, Mariscal Brice Tchatat Tali, Lauve Rachel Tchokouaha Yamthe, Bill Baker, Fabrice Fekam Boyom

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Article in Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Christelle Wayoue KomDepartment of Biochemistry, University of Yaoundé I, Yaoundé, Cameroon.
Sylvester Osei BobbieDepartment of Chemistry, University of South Florida, Tampa, USA.
Darline DizeDepartment of Biochemistry, University of Yaoundé I, Yaoundé, Cameroon.
Cyrille Armel Njanpa NgansopDepartment of Biochemistry, University of Yaoundé I, Yaoundé, Cameroon.
Mariscal Brice Tchatat TaliDepartment of Biochemistry, University of Yaoundé I, Yaoundé, Cameroon.
Lauve Rachel Tchokouaha YamtheDepartment of Biochemistry, University of Yaoundé I, Yaoundé, Cameroon. yamthe_lauve@yahoo.fr.
Bill BakerDepartment of Chemistry, University of South Florida, Tampa, USA.
Fabrice Fekam BoyomDepartment of Biochemistry, University of Yaoundé I, Yaoundé, Cameroon. fabrice.boyom@fulbrightmail.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGardenia imperialis K. Schum. is a medicinal plant used in traditional medicine to treat malaria and related symptoms. This bio-guided study aimed at identifying antiparasitic compounds from G. imperialis against the causative agents of malaria (Plasmodium falciparum), visceral leishmaniasis (Leishmania donovani), and sleeping sickness (Trypanosoma brucei brucei).

methodsCrude methanolic extracts from the leaf and stem bark of G. imperialis were studied using liquid chromatography-mass spectrometry (LC-MS) and preparative medium/high-performance liquid chromatography (M/HPLC) guided by evidence of antiparasitic activity. The structures of the isolated compounds were elucidated using nuclear magnetic resonance (NMR) and MS. All the extracts, fractions, subfractions, and pure compounds were evaluated for antiparasitic activity. Cytotoxicity was tested against Vero and Raw264.7 mammalian cell lines.

resultsThe leaf methanolic extract (GIlMeOH) exhibited broad-spectrum antiparasitic activity (IC₅₀ < 11 µg/mL) and low cytotoxicity (median cell cytotoxicity-CC

conclusionThe promising antiparasitic profile of the G. imperialis leaf extract and its isolated constituents, particularly and 5,7,3'-trihydroxy-6,4',5'-trimethoxyflavone (2), gradenin A (3), and salvigenin (4), warrants further investigation into their potential as novel and selective agents against the target parasitic diseases.

Indexed as

Antiparasitic AgentsGardeniaPlant ExtractsAnimalsChlorocebus aethiopsChromatography, High Pressure LiquidLeishmania donovaniMass SpectrometryMicePlant BarkPlant LeavesPlasmodium falciparumRAW 264.7 CellsTrypanosoma brucei bruceiVero CellsAntiparasitic AgentsPlant ExtractsAntiparasitic activityFlavonoidsGardenia imperialisMalariaSleeping sicknessVisceral leishmaniasis

Identifiers

PMID42315821
PMCPMC13280111

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