ArticleScientific reports2026
Electrospun poly(lactic acid) biopolymer fibers functionalized with Y-sperm-specific scFv enable immunoaffinity-driven bovine sperm sexing.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Assisted reproductive technologies increasingly demand efficient approaches for sperm sexing in livestock, ideally compatible with scalable manufacturing and reduced resource use. This study serves as a proof-of-concept for a biocompatible electrospun poly (lactic acid) (PLA) biomaterial platform functionalized with a recombinant single-chain variable fragment antibody specific to bovine Y-sperm (BY-scFv), enabling selective separation of bovine Y-sperm from X-sperm populations. Electrospun mats were prepared by selection PLA concentration (6-10% w/w) and deposited solution volume (10-20 mL), and were evaluated based on electrospinning performance, fiber morphology, thickness, water-accessible porosity and water uptake capacity. A mat fabricated at 8% PLA with a 15 mL deposited volume was selected for subsequent functionalization and separation experiments. The presence and retention of immobilized BY-scFv after EDC/NHS coupling were supported by ELISA, FT‑IR, SEM, and fluorescence labeling. Using a syringe‑packed format, the functionalized mats enabled selective sperm capture and produced sex sperm ratio in different fractions, with maximum values of 71.4% X‑sperm in the supernatant fraction and 76.1% Y‑sperm in the eluted fraction. These results indicate that antibody‑functionalized electrospun PLA scaffolds can serve as a biopolymer-based platform for sperm immunoaffinity processing, with potential for further optimization and translational evaluation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.