ArticleJournal of veterinary internal medicine2026
Steroidal response following intravenous administration of long-term frozen tetracosactide acetate in healthy Beagles.
Article in Journal of veterinary internal medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
backgroundThe adrenocorticotropic hormone (ACTH) stimulation test is used to assess adrenal function. The effects of extended freezing of tetracosactide acetate (TCA), an ACTH analogue, on test performance, remain unexplored. HYPOTHESIS/
objectivesTo determine whether TCA retains its biological activity to induce adrenal steroid production when stored in plastic syringes and frozen at -20 °C for prolonged period. ANIMALS: Eight adult experimental Beagles divided into 2 groups of 2 males and 2 females each.
methodsProspective case-crossover study. Each dog received 5 μg/kg IV of TCA on 2 occasions, 4 weeks apart (period P1-washout-period P2). Group 1 received frozen then fresh TCA; group 2 received fresh then frozen TCA. Pre- and post-administration blood samples were analyzed at both study periods for cortisol and other steroid metabolites using liquid chromatography-tandem mass spectrometry. A general linear mixed model was used with TCA (fresh vs frozen), period, sequence and timing as fixed factors, and dog as random factor.
resultsFrozen TCA stored for a median time of 1.8 years (IQ range 1.5-1.9) induced similar steroid metabolite responses compared with fresh TCA. Median T1 cortisol concentrations were 248 nmol/L (IQ range 231-272) with frozen TCA and 254 nmol/L (IQ range 249-262) with fresh TCA (P = .037). Median T1 17-OHP concentrations were 0.73 μg/L (IQ range 0.67-0.85) with frozen TCA 0.79 μg/L (IQ range 0.71-1.17) with fresh TCA (P = .09). CONCLUSIONS AND CLINICAL IMPORTANCE: Long-term freezing of TCA in plastic syringes offers a cost-effective strategy and practical alternative during supply shortages.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.