Evidence map›Paper›PMID 42314039›Full record

ArticleBlood advances2026

Plasmin-mediated fibrinolysis is required for hematopoietic recovery after 5-FU-induced myeloablation.

Tiffany P Nguyen, Yong G Jiang, Betul M Ogan, Andrew D Doyle, Duy T Tran, Jerrold M Ward, Andrew Zhang, Rafaela Ferrer de Oliveira, Francesca Ferraresso, Jacob R Bledsoe and 5 more

Abstract read
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In one paragraph

Article in Blood advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Tiffany P NguyenProteases and Tissue Remodeling Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.
Yong G JiangProteases and Tissue Remodeling Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.ORCID 0009-0006-7510-6987
Betul M OganDepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Harvard University, Boston, MA.ORCID 0009-0001-8591-0841
Andrew D DoyleImaging Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.ORCID 0000-0002-0442-5415
Duy T TranImaging Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.
Jerrold M WardProteases and Tissue Remodeling Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.ORCID 0000-0002-6350-8982
Andrew ZhangProteases and Tissue Remodeling Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.ORCID 0000-0001-5594-4664
Rafaela Ferrer de OliveiraDepartment of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Harvard University, Boston, MA.ORCID 0000-0001-6572-5895
Francesca FerraressoVersiti Blood Research Institute, Milwaukee, WI.
Jacob R BledsoeDepartment of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA.ORCID 0000-0002-2387-5462
Christian J KastrupVersiti Blood Research Institute, Milwaukee, WI.
Matthew J FlickBlood Research Center, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC.ORCID 0000-0002-5034-3162
R Grant RoweDepartment of Hematology/Oncology, Boston Children's Hospital and Harvard Medical School, Boston, MA.
Thomas H BuggeProteases and Tissue Remodeling Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.
Lakmali M SilvaProteases and Tissue Remodeling Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD.ORCID 0000-0001-6282-2657

Funding

Serine Proteases In Remodeling Of The Extracellular MatrZ01DE000699 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI BUGGE, THOMAS H · 2000 to 2008
$3.2M
Intramural NIH HHS Z01 DE000699Intramural NIH HHS Z01-DE0699
6 · The paper itself

Abstract

abstractHematopoietic recovery after myeloablation plays a critical role in the survival of patients who receive chemotherapy or hematopoietic stem cell transplantation. Understanding the mechanisms underlying hematopoiesis helps identify the molecular and/or cellular determinants that could be targeted to enhance hematopoietic recovery. Although several mechanisms by which the plasminogen (Plg) activation system promotes hematopoietic recovery after myelosuppression have been reported, the specific role fibrinolysis plays in hematopoietic recovery is unclear. The current study employs 5-fluorouracil-mediated myeloablation in murine models and provides a clear demonstration of uniform extravascular fibrin deposits in the bone marrow (BM) niche upon myeloablation. Persistent fibrin deposits in Plg-deficient mice lead to failure of hematopoietic recovery. Genetic and pharmacological depletion of fibrinogen after myeloablation restored hematopoietic recovery, revealing the critical role fibrin plays in this process. Moreover, defective fibrinolysis led to delayed hematopoietic recovery via a mechanism that involves fibrin engagement with the major myeloid integrin αMβ2. Importantly, similar fibrin-rich marrow niches were also observed in human BM injury and failure states. The study identifies a novel molecular pathway that could be therapeutically targeted to enhance hematopoietic recovery after myeloablation.

Indexed as

FibrinolysinFibrinolysisFluorouracilHematopoiesisAnimalsBone MarrowFibrinHematopoietic Stem CellsHumansMicePlasminogenFibrinFibrinolysinFluorouracilPlasminogen

Identifiers

PMID42314039
PMCPMC13578982

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.