Evidence map›Paper›PMID 42312885›Full record

ArticleACS chemical biology2026

NanoBRET Saturation Analysis with a Novel HaloTag Ligand Reveals Butyrophilin Heteromeric Interactions Controlling γ9δ2 T Cell Activation.

Girija Pawge, Sidra Bashir, Ritika Parikh, Chia-Hung Christine Hsiao, Andrew J Wiemer

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In one paragraph

Article in ACS chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Girija PawgeDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269, United States.ORCID 0000-0002-7155-0777
Sidra BashirDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269, United States.
Ritika ParikhDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269, United States.
Chia-Hung Christine HsiaoDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269, United States.
Andrew J WiemerDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269, United States.ORCID 0000-0002-3029-1780

Funding

Role of immune modulating butyrophilins in gamma delta T cell activationR01AI150869 · NIAID · UNIVERSITY OF CONNECTICUT STORRS · PI VINOGRADOVA, OLGA, WIEMER, ANDREW J · 2020 to 2024
$2.0M
Synthesis and evaluation of BTN3A1 ligands for cancer immunotherapyR01CA266138 · NCI · UNIVERSITY OF CONNECTICUT STORRS · PI ANDREW J WIEMER · 2022 to 2026
$1.8M
NCI NIH HHS R01 CA266138NIAID NIH HHS R01 AI150869
6 · The paper itself

Abstract

Human γ9δ2 T cells detect microbial phosphoantigens (pAg) through butyrophilin (BTN) family proteins; however, how multiple BTN paralogs assemble into functional signaling complexes remains unresolved. Because γ9δ2 T cell activation requires coordinated action of several BTN members rather than a single receptor pair, defining their organization in living cells is essential. Here, we apply a live-cell NanoBRET saturation analysis using a novel HaloTag ligand to quantitatively resolve BTN oligomerization dynamics. We found that BTN3A1 exists as higher-order basal oligomers. Coexpression of BTN3A2 or BTN3A3 redistributes these assemblies into defined BTN3 dimers. The BTN3 dimers have a baseline interaction with BTN2A1 dimers that is altered by IgV domain interactions and by classical pAgs such as (

Indexed as

Antigens, CDButyrophilinsLymphocyte ActivationT-LymphocytesHumansLigandsOrganophosphatesProtein Multimerization4-hydroxy-3-methyl-2-butenyl diphosphateAntigens, CDBTN3A1 protein, humanButyrophilinsLigandsOrganophosphates

Identifiers

PMID42312885
PMCPMC13322128

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.