Evidence map›Paper›PMID 42312181›Full record

ArticleISME communications2026

Revised 16S rRNA V4 hypervariable region targeting primers enhance detection of Patescibacteria and other lineages across diverse environments.

Huifeng Hu, Clemens Karwautz, Kalina Duszka, Thomas Karner, Isabella C Wagner, Christoph Grander, Wilhelm Grander, Laura Steinwidder, Lucilla Boito, Viktor Van de Velde and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ISME communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Huifeng HuCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.ORCID https://orcid.org/0000-0001-5543-8390
Clemens KarwautzDepartment of Functional and Evolutionary Ecology, Faculty of Life Sciences, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.
Kalina DuszkaDepartment of Nutritional Sciences, Faculty of Lifesciences, University of Vienna, Josef-Holaubek-Platz 2, Vienna 1090, Austria.
Thomas KarnerFaculty of Psychology, University of Vienna, Liebiggasse 5, Vienna 1010, Austria.
Isabella C WagnerCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.
Christoph GranderDepartment of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology, and Metabolism, Medical University of Innsbruck, Innrain 52, Innsbruck 6020, Austria.
Wilhelm GranderDepartment of Internal Medicine, Hall State Hospital, Milserstrasse 10, Hall in Tirol 6060, Austria.
Laura SteinwidderBiobased Sustainability Engineering (SUSTAIN), Department of Bioscience Engineering, University of Antwerp, Antwerp 2020, Belgium.
Lucilla BoitoBiobased Sustainability Engineering (SUSTAIN), Department of Bioscience Engineering, University of Antwerp, Antwerp 2020, Belgium.
Viktor Van de VeldeDepartment of Environment, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Gent 9000, Belgium.
Marijn BautersDepartment of Environment, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Gent 9000, Belgium.
Pascal BoeckxDepartment of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Gent 9000, Belgium.
David SekiCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.
Bettina GlaslCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.ORCID https://orcid.org/0000-0002-6812-868X
Stefan ThieleCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.
Hannes SchmidtCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.ORCID https://orcid.org/0000-0003-4288-4257
Joana SénecaCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.ORCID https://orcid.org/0000-0003-3951-3674
Michael WagnerCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.ORCID https://orcid.org/0000-0002-9778-7684
Petra PjevacCentre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna 1030, Austria.ORCID https://orcid.org/0000-0001-7344-302X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Primer bias in 16S rRNA gene amplicon sequencing can distort microbial diversity estimates by underrepresenting key taxa. We introduce a modified primer pair (V4-EXT) targeting the hypervariable V4 region of bacterial and archaeal 16S rRNA genes, with improved

Indexed as

16S rRNA gene amplicon sequencingPatescibacteriaprimer modification

Identifiers

PMID42312181
PMCPMC13271367

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.