ArticleJournal of the science of food and agriculture2026
Environmental persistence of fluoroquinolones: Reverse-phase high-performance liquid chromatography-based surveillance of contamination in water, soil, and food sources.
Article in Journal of the science of food and agriculture, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundFluoroquinolone (FQ) antibiotics persist in the environment, posing risks to ecosystems and public health through antimicrobial resistance and bioaccumulation. Effective analytical methods are essential for monitoring these residues and enforcing regulations to limit antibiotic pollution.
resultsThis study developed validated reversed-phase high-performance liquid chromatography (RP-HPLC) approaches for the simultaneous detection of five FQs: norfloxacin (NFX), levofloxacin (LFX), enrofloxacin (ENR), ofloxacin (OFX), and ciprofloxacin (CFX). Two chromatographic methods were required due to structural similarities between LFX and OFX. Method I, using an Eclipse Plus phenyl hexyl column, produced retention times of 5.2 min for NFX, 5.7 min for LFX, and 7.9 min for ENR. Method II, utilizing a YMC C18 column, yielded retention times of 8.9 min for OFX and 10.7 min for CFX. Both methods demonstrated high selectivity, specificity, linearity (R
conclusionThe validated RP-HPLC methods provide robust tools for monitoring FQ residues in diverse matrices. The widespread contamination observed highlights the urgent need for stronger surveillance, stricter regulatory enforcement, and comprehensive risk assessment to mitigate antimicrobial resistance and protect public health. © 2026 Society of Chemical Industry.
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